Article (Scientific journals)
Fingerprint profiling of polysaccharide kefiran extracted from kefir grains biomass
Pop, Carmen; Rotar, Ancuta M.; Salanțӑ, Liana C. et al.
2015In Journal of Agroalimentary Processes and Technologies, 21 (2), p. 207-212
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Keywords :
kefiran; monosaccharide; HPTLC; kefir grains
Abstract :
[en] Kefiran is a water-soluble polysaccharide extracted from kefir grains biomass. The identification and quantification of kefiran monosaccharides were carried out by HPTLC after the complete acid hydrolysis of kefiran solutions. The mobile phase was a mixture of n-propanol: acetic acid: water (70:20:10, v/v). For derivatization was used p-aminobenzoic acid and o-phosphoric acid in methanol. The identified HPTLC fractions were glucose (Rf 0.71) and galactose (Rf 0.66), which indicates a high purity of the extracted kefiran. The relative concentrations of each monosaccharide identified in samples are dependent on the initial molecular weight of the polymer chain. The kefiran isolated from kefir grains grown in milk is a heteropolysaccharide which contains D-glucose and D-galactose units in a ratio of 0.94:1.1.
Disciplines :
Food science
Author, co-author :
Pop, Carmen;  University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5, Mănăştur Street, 400372 Cluj-Napoca, Romania > Faculty of Food Science and Technology
Rotar, Ancuta M.;  University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5, Mănăştur Street, 400372 Cluj-Napoca, Romania > Faculty of Food Science and Technology
Salanțӑ, Liana C.;  University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5, Mănăştur Street, 400372 Cluj-Napoca, Romania > Faculty of Food Science and Technology
Semeniuc, Cristina A.;  University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5, Mănăştur Street, 400372 Cluj-Napoca, Romania > Faculty of Food Science and Technology
Socaciu, Carmen;  University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5, Mănăştur Street, 400372 Cluj-Napoca, Romania > Faculty of Food Science and Technology
Sindic, Marianne  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Laboratoire Qualité et sécurité des produits agro-aliment.
Language :
English
Title :
Fingerprint profiling of polysaccharide kefiran extracted from kefir grains biomass
Publication date :
29 June 2015
Journal title :
Journal of Agroalimentary Processes and Technologies
ISSN :
2069-0053
eISSN :
2068-9551
Publisher :
Faculty of Food Processing Technology, Romania
Volume :
21
Issue :
2
Pages :
207-212
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 June 2016

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