Article (Scientific journals)
Use of Estetrol with other Steroids for Attenuation of Neonatal Hypoxic-Ischemic brain injury:to combine or not to combine?
Tskitishvili, Ekaterine; Pequeux, Christel; Munaut, Carine et al.
2016In Oncotarget
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Keywords :
neonatal hypoxic-ischemic encephalopathy; hippocampus; cortex; estetrol; estradiol
Abstract :
[en] Estetrol (E4), estradiol (E2) and progesterone (P4) have important antioxidative and neuroprotective effects in neuronal system. We aimed to study the consequence of combined steroid therapy in neonatal hypoxic-ischemic encephalopathy (HIE). In vitro the effect of E4 combined with other steroids on oxidative stress and the cell viability in primary hippocampal cultures was evaluated by lactate dehydrogenase and cell survival assays. In vivo neuroprotective and therapeutic efficacy of E4 combined with other steroids was studied in HIE model of immature rats. The rat pups rectal temperature, body and brain weights were evaluated. The hippocampus and the cortex were investigated by histo/immunohistochemistry: intact cell number counting, expressions of markers for early gray matter lose, neuro- and angiogenesis were studied. Glial fibrillary acidic protein was evaluated by ELISA in blood samples. In vitro E4 and combinations of high doses of E4 with P4 and/or E2 significantly diminished the LDH activity and upregulated the cell survival. In vivo pretreatment or treatment by different combinations of E4 with other steroids had unalike effects on body and brain weight, neuro- and angiogenesis, and GFAP expression in blood. The combined use of E4 with other steroids has no benefit over the single use of E4.
Disciplines :
Pediatrics
Neurology
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Tskitishvili, Ekaterine  ;  Université de Liège > GIGA-Research
Pequeux, Christel  ;  Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Munaut, Carine  ;  Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
VIELLEVOYE, Renaud ;  Centre Hospitalier Universitaire de Liège - CHU > Service néonatologie (CHR)
Nisolle, Michelle ;  Université de Liège > Département des sciences cliniques > Gynécologie - Obstétrique
Noël, Agnès ;  Université de Liège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Foidart, Jean-Michel ;  Université de Liège > Département des sciences cliniques > Département des sciences cliniques
Language :
English
Title :
Use of Estetrol with other Steroids for Attenuation of Neonatal Hypoxic-Ischemic brain injury:to combine or not to combine?
Publication date :
26 May 2016
Journal title :
Oncotarget
eISSN :
1949-2553
Publisher :
Impact Journals, Albany, United States - New York
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 May 2016

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