Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC

Fakih, Ibrahim; Thiry, Damien; Duprez, Jean-Noël; Saulmont, Marc; Iguchi, A.; Piérard, Denis; Jouant, Ludovic; Daube, Georges; Ogura, Y.; Hayashi, T.; Taminiau, Bernard; Mainil, Jacques

doi:10.1016/j.vetmic.2016.02.017

Article (Scientific journals)

Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC

Fakih, Ibrahim; Thiry, Damien; Duprez, Jean-Noël et al.

2017 • In Veterinary Microbiology, 202, p. 16-22

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/197014

DOI
10.1016/j.vetmic.2016.02.017

PubMed
26923249

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Keywords :

AE-STEC; calf diarrhoea; EPEC; 05 serogroup; whole genome sequencing

Abstract :

[en] Escherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC) producing the AE lesion only cause non-bloody diarrhoea in all mammals. The purpose of this study was (i) to identify the pathotypes of enterohaemolysin-producing E. coli isolated between 2009 and 2013 on EHLY agar from less than 2 month-old diarrhoeic calves with a triplex PCR targeting the stx1, stx2, eae virulence genes; (ii) to serotype the positive isolates with PCR targeting the genes coding for ten most frequent and pathogenic human and calf STEC O serogroups; and (iii) to compare the MLSTypes and virulotypes of calf and human O5 AE-STEC after Whole Genome Sequencing using two server databases (www.genomicepidemiology.org). Of 233 isolates, 206 were triplex PCR-positive: 119 AE-STEC (58%), 78 EPEC (38%) and 9 STEC (4%); and the stx1+eae+ AE-STEC (49.5%) were the most frequent. Of them, 120 isolates (84% of AE-STEC, 23% of EPEC, 22% of STEC) tested positive with one O serogroup PCR: 57 for O26 (47.5%), 36 for O111 (30%), 10 for O103 (8%) and 8 for O5 (7%) serogroups. The analysis of the draft sequences of 15 O5 AE-STEC could not identify any difference correlated to the host. As a conclusion, (i) the AE-STEC associated with diarrhoea in young calves still belong to the same serogroups as previously (O5, O26, O111) but the O103 serogroup may be emerging, (ii) the O5 AE-STEC from calves and humans are genetically similar

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Fakih, Ibrahim ^✱

Thiry, Damien ^✱; Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes

Duprez, Jean-Noël ; Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Département des maladies infectieuses et parasitaires (DMI)

Saulmont, Marc

Iguchi, A.

Piérard, Denis

Jouant, Ludovic

Daube, Georges ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Ogura, Y.

Hayashi, T.

Taminiau, Bernard ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Mainil, Jacques ; Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes

^✱ These authors have contributed equally to this work.

Language :

English

Title :

Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC

Publication date :

April 2017

Journal title :

Veterinary Microbiology

ISSN :

0378-1135

eISSN :

1873-2542

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

202

Pages :

16-22

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 May 2016

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