Volumetric Absorptive Microsampling for Hepcidin Peptide Extraction from Whole Blood

Houbart, Virginie; COBRAIVILLE, Gaël; Nys, Gwenaël; Servais, Anne-Catherine; Fillet, Marianne

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Volumetric Absorptive Microsampling for Hepcidin Peptide Extraction from Whole Blood

Houbart, Virginie; COBRAIVILLE, Gaël; Nys, Gwenaël et al.

2016 • In LC GC North America, 34 (5)

Peer reviewed

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https://hdl.handle.net/2268/196988

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Abstract :

[en] Whole blood analysis is an emerging trend in the field of bioanalysis. We developed a fast and simple protocol to extract and analyze a peptide, hepcidin, from whole blood. Sampling and extraction were carried out using volumetric absorptive microsampling (VAMS), a novel blood collection method that allows the sampling of a known blood volume independently from hematocrit. The composition of the extraction medium was optimized using an experimental design to get the most intense signal of hepcidin, considering different organic solvents and acidic additives.

Research Center/Unit :

Centre Interfacultaire de Recherche du Médicament - CIRM

Disciplines :

Pharmacy, pharmacology & toxicology

Author, co-author :

Houbart, Virginie ; Université de Liège > Département de pharmacie > Analyse des médicaments

COBRAIVILLE, Gaël ; Centre Hospitalier Universitaire de Liège - CHU > Service de rhumatologie

Nys, Gwenaël ; Université de Liège > Département de pharmacie > Analyse des médicaments

Servais, Anne-Catherine ; Université de Liège > Département de pharmacie > Analyse des médicaments

Fillet, Marianne ; Université de Liège > Département de pharmacie > Analyse des médicaments

Language :

English

Title :

Volumetric Absorptive Microsampling for Hepcidin Peptide Extraction from Whole Blood

Publication date :

May 2016

Journal title :

LC GC North America

ISSN :

1527-5949

eISSN :

1939-1889

Publisher :

UBM Advanstar Communications, Duluth, United States - Minnesota

Volume :

Issue :

Peer reviewed :

Peer reviewed

Available on ORBi :

since 19 May 2016

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