Article (Scientific journals)
Fluorescent reporter libraries as useful tools for optimizing microbial cell factories: a review of the current methods and applications
Delvigne, Frank; Pêcheux, Hélène; Tarayre, Cédric
2015In Frontiers in Bioengineering and Biotechnology, 3
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Keywords :
mini-bioreactors; high-throughput; flow cytometry; single cell
Abstract :
[en] The use of genetically encoded fluorescent reporters allows speeding up the initial optimization steps of microbial bioprocesses. These reporters can be used for determining the expression level of a particular promoter, not only the synthesis of a specific protein but also the content of intracellular metabolites. The level of protein/metabolite is thus proportional to a fluorescence signal. By this way, mean expression profiles of protein/ metabolites can be determined non-invasively at a high-throughput rate, allowing the rapid identification of the best producers. Actually, different kinds of reporter systems are available, as well as specific cultivation devices allowing the on-line recording of the fluorescent signal. Cell-to-cell variability is another important phenomenon that can be integrated into the screening procedures for the selection of more efficient microbial cell factories.
Disciplines :
Biotechnology
Author, co-author :
Delvigne, Frank  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Pêcheux, Hélène
Tarayre, Cédric ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Language :
English
Title :
Fluorescent reporter libraries as useful tools for optimizing microbial cell factories: a review of the current methods and applications
Publication date :
28 September 2015
Journal title :
Frontiers in Bioengineering and Biotechnology
eISSN :
2296-4185
Publisher :
Frontiers Research Foundation, Switzerland
Volume :
3
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 February 2016

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