mini-bioreactors; high-throughput; flow cytometry; single cell
Abstract :
[en] The use of genetically encoded fluorescent reporters allows speeding up the initial optimization
steps of microbial bioprocesses. These reporters can be used for determining
the expression level of a particular promoter, not only the synthesis of a specific protein
but also the content of intracellular metabolites. The level of protein/metabolite is thus
proportional to a fluorescence signal. By this way, mean expression profiles of protein/
metabolites can be determined non-invasively at a high-throughput rate, allowing the
rapid identification of the best producers. Actually, different kinds of reporter systems
are available, as well as specific cultivation devices allowing the on-line recording of the
fluorescent signal. Cell-to-cell variability is another important phenomenon that can be
integrated into the screening procedures for the selection of more efficient microbial cell
factories.
Disciplines :
Biotechnology
Author, co-author :
Delvigne, Frank ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Pêcheux, Hélène
Tarayre, Cédric ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Language :
English
Title :
Fluorescent reporter libraries as useful tools for optimizing microbial cell factories: a review of the current methods and applications
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