Histopathological effects of Aspergillus clavatus (Ascomycota: Trichocomaceae) on larvae of the southern house mosquito, Culex quinquefasciatus (Diptera: Culicidae)
Microbial control; Entomopathogenic fungi; Aspergillosis; Bioassay; Histopathology; Electron microscopy
Abstract :
[en] Aspergillus clavatus (Ascomycota: Trichocomaceae) was previously found to be an opportunistic pathogen of mosquitoes (Diptera: Culicidae). In the present study, the mechanism leading to its insecticidal activity was investigated regarding histological damages on Culex quinquefasciatus larvae exposed to A. clavatus spores. Multiple concentration assays using spore suspensions (0.5 x 10^8 to 2.5 x 10^8 spores/ml) revealed 17.0% to 74.3% corrected mortalities after 48 h exposure. Heat-deactivated spores induced a lower mortality compared to non-heated spores suggesting that insecticidal effects are actively exerted. Spore-treated and untreated larvae were prepared for light microscopy as well as for scanning and transmission electron microscopy. Spores failed to adhere to the external body surface (except the mouth parts) of these aquatic immature stages but progressively filled the digestive tract where their metabolism seemed to activate. In parallel, the internal tissues of the larvae, i.e. the midgut wall, the skeletal muscles, and the cuticle-secreting epidermis, were progressively destroyed between 8 and 24 h of exposure. These observations suggest that toxins secreted by active germinating spores of A. clavatus in the digestive tract altered the larval tissues, leading to their necrosis and causing larval death. Fungal proliferation and sporulation then occurred during a saprophytic phase. A. clavatus enzymes or toxins responsible for these pathogenic effects need to be identified in further studies before any use of this fungus in mosquito control.
Disciplines :
Microbiology Entomology & pest control
Author, co-author :
Bawin, Thomas ; Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Seye, Fawrou; Université Cheik Anta Diop > Faculté des Sciences et Techniques > Laboratoire de Biologie de la Reproduction
Zimmer, Jean-Yves ; Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Raharimalala, Fara Nantenaina; Institut Pasteur de Madagascar > Unité d'entomologie médicale
Ndiaye, Mady; University Cheik Anta Diop > Faculté des Sciences et Techniques > Laboratoire de Biologie de la Reproduction
Compère, Philippe ; Université de Liège > Département de Biologie, Ecologie et Evolution > Morphologie fonctionnelle et évolutive
Delvigne, Frank ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Francis, Frédéric ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Histopathological effects of Aspergillus clavatus (Ascomycota: Trichocomaceae) on larvae of the southern house mosquito, Culex quinquefasciatus (Diptera: Culicidae)
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