Article (Scientific journals)
KPC2 relocalizes HOXA2 to the cytoplasm and decreases its transcriptional activity.
Bridoux, Laure; Bergiers, Isabelle; Draime, Amandine et al.
2015In Biochimica et Biophysica Acta, 1849 (10), p. 1298-311
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Keywords :
Animals; Cytoplasm/metabolism; Embryonic Development/genetics; Gene Expression Regulation, Developmental; Homeodomain Proteins/biosynthesis/genetics/metabolism; Intracellular Signaling Peptides and Proteins/genetics; Mice; Protein Binding; Transcriptional Activation/genetics; HOX; HOXA2; Interaction; KPC2; Mouse development
Abstract :
[en] Regulation of transcription factor activity relies on molecular interactions or enzymatic modifications which influence their interaction with DNA cis-regulatory sequences, their transcriptional activation or repression, and stability or intracellular distribution of these proteins. Regarding the well-conserved Hox protein family, a restricted number of activity regulators have been highlighted thus far. In the framework of a proteome-wide screening aiming at identifying proteins interacting with Hoxa2, KPC2, an adapter protein constitutive of the KPC ubiquitin-ligase complex, was identified. In this work, KPC2 was confirmed as being a genuine interactor of Hoxa2 by co-precipitation and bimolecular fluorescence complementation assays. At functional level, KPC2 diminishes the transcriptional activity and induces the nuclear exit of Hoxa2. Gene expression analyses revealed that Kpc2 is active in restricted areas of the developing mouse embryo which overlap with the Hoxa2 expression domain. Together, our data support that KPC2 regulates Hoxa2 by promoting its relocation to the cytoplasm.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Bridoux, Laure
Bergiers, Isabelle
Draime, Amandine
Halbout, Mathias
Deneyer, Noemie
Twizere, Jean-Claude  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Biologie cell. et moléc.
Rezsohazy, Rene
Language :
English
Title :
KPC2 relocalizes HOXA2 to the cytoplasm and decreases its transcriptional activity.
Publication date :
2015
Journal title :
Biochimica et Biophysica Acta
ISSN :
0006-3002
eISSN :
1878-2434
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
1849
Issue :
10
Pages :
1298-311
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2015 Elsevier B.V. All rights reserved.
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