Article (Scientific journals)
Simultaneous production of acetic and gluconic acids by a thermotolerant Acetobacter strain during acetous fermentation in a bioreactor
Mounir, Majid; Shafiei, rasoul; Zarmehrkhorshid, Raziyeh et al.
2016In Journal of Bioscience and Bioengineering, 121 (2), p. 166-171
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Keywords :
Acetobacter; Thermoresistant; Bioreactor; Enzyme activity; Acetic acid; Gluconic acid
Abstract :
[en] The activity of bacterial strains significantly influences the quality and the taste of vinegar. Previous studies of acetic acid bacteria have primarily focused on the ability of bacterial strains to produce high amounts of acetic acid. However, few studies have examined the production of gluconic acid during acetous fermentation at high temperatures. The production of vinegar at high temperatures by two strains of acetic acid bacteria isolated from apple and cactus fruits, namely AF01 and CV01, respectively, was evaluated in this study. The simultaneous production of gluconic and acetic acids was also examined in this study. Biochemical and molecular identification based on a 16s rDNA sequence analysis confirmed that these strains can be classified as Acetobacter pasteurianus. To assess the ability of the isolated strains to grow and produce acetic acid and gluconic acid at high temperatures, a semi-continuous fermentation was performed in a 20-L bioreactor. The two strains abundantly grew at a high temperature (41°C). At the end of the fermentation, the AF01 and CV01 strains yielded acetic acid concentrations of 7.64% (w/v) and 10.08% (w/v), respectively. Interestingly, CV01 was able to simultaneously produce acetic and gluconic acids during acetic fermentation, whereas AF01 mainly produced acetic acid. In addition, CV01 was less sensitive to ethanol depletion during semi-continuous fermentation. Finally, the enzymatic study showed that the two strains exhibited high ADH and ALDH enzyme activity at 38°C compared with the mesophilic reference strain LMG 1632, which was significantly susceptible to thermal inactivation.
Disciplines :
Microbiology
Biotechnology
Genetics & genetic processes
Author, co-author :
Mounir, Majid ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Shafiei, rasoul
Zarmehrkhorshid, Raziyeh 
Hamouda, Allal
Ismaili Alaoui, Mustapha
Thonart, Philippe ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Language :
English
Title :
Simultaneous production of acetic and gluconic acids by a thermotolerant Acetobacter strain during acetous fermentation in a bioreactor
Publication date :
February 2016
Journal title :
Journal of Bioscience and Bioengineering
ISSN :
1389-1723
eISSN :
1347-4421
Publisher :
Elsevier Science, Osaka, Japan
Volume :
121
Issue :
2
Pages :
166-171
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
CTB - Coopération Technique Belge [BE]
Available on ORBi :
since 16 December 2015

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