Biochemical characterization of the Pseudomonas aeruginosa 101/1477 metallo-beta-lactamase IMP-1 produced by Escherichia coli.

Laraki, N.; Franceschini, N.; Rossolini, G. M.; Santucci, P.; Meunier, C.; De Pauw, Edwin; Amicosante, G.; Frère, Jean-Marie; Galleni, Moreno

doi:10.1128/aac.43.4.902

Article (Scientific journals)

Biochemical characterization of the Pseudomonas aeruginosa 101/1477 metallo-beta-lactamase IMP-1 produced by Escherichia coli.

Laraki, N.; Franceschini, N.; Rossolini, G. M. et al.

1999 • In Antimicrobial Agents and Chemotherapy, 43 (4), p. 902-6

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/188988

DOI
10.1128/aac.43.4.902

PubMed
10103197

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Keywords :

Anti-Bacterial Agents/metabolism; Bacterial Proteins; Chelating Agents/pharmacology; Escherichia coli/genetics/metabolism; Humans; Hydrogen-Ion Concentration; Kinetics; Picolinic Acids/pharmacology; Pseudomonas aeruginosa/enzymology; Zinc/metabolism; beta-Lactamase Inhibitors; beta-Lactamases/metabolism

Abstract :

[en] The blaIMP gene coding for the IMP-1 metallo-beta-lactamase produced by a Pseudomonas aeruginosa clinical isolate (isolate 101/1477) was overexpressed via a T7 expression system in Escherichia coli BL21 (DE3), and its product was purified to homogeneity with a final yield of 35 mg/liter of culture. The structural and functional properties of the enzyme purified from E. coli were identical to those of the enzyme produced by P. aeruginosa. The IMP-1 metallo-beta-lactamase exhibits a broad-spectrum activity profile that includes activity against penicillins, cephalosporins, cephamycins, oxacephamycins, and carbapenems. Only monobactams escape its action. The enzyme activity was inhibited by metal chelators, of which 1,10-o-phenanthroline and dipicolinic acid were the most efficient. Two zinc-binding sites were found. The zinc content of the P. aeruginosa 101/1477 metallo-beta-lactamase was not pH dependent.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Laraki, N.

Franceschini, N.

Rossolini, G. M.

Santucci, P.

Meunier, C.

De Pauw, Edwin ; Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Amicosante, G.

Frère, Jean-Marie ; Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines

Galleni, Moreno ; Université de Liège - ULiège

Language :

English

Title :

Biochemical characterization of the Pseudomonas aeruginosa 101/1477 metallo-beta-lactamase IMP-1 produced by Escherichia coli.

Publication date :

1999

Journal title :

Antimicrobial Agents and Chemotherapy

ISSN :

0066-4804

eISSN :

1098-6596

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

902-6

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 December 2015

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