The bla gene of the cephamycin cluster of Streptomyces clavuligerus encodes a class A beta-lactamase of low enzymatic activity.

Amino Acid Sequence; Anti-Bacterial Agents/biosynthesis/metabolism; Base Sequence; Cephamycins/biosynthesis; Cloning, Molecular; Genes, Bacterial; Kinetics; Molecular Sequence Data; Molecular Weight; Penicillin G/metabolism; Recombinant Proteins/metabolism; Streptomyces/enzymology/genetics; Transformation, Genetic; beta-Lactamases/chemistry/genetics/metabolism

Abstract :

[en] A gene (bla) encoding a beta-lactamase is present in the cephamycin gene cluster of Streptomyces clavuligerus, the strain producing clavulanic acid and a beta-lactamase inhibitory protein. The bla gene is located 5.1 kb downstream from and in the opposite orientation to cefE, encoding the deacetoxycephalosporin C synthase. The bla gene encodes a 332-residue protein (Mr, 35,218), similar to other class A beta-lactamases produced by actinomycetes. Modification (to SDG) of the SDN conserved motif of class A beta-lactamases as well as of amino acids in otherwise conserved regions in the molecule may explain the low penicillinase and cephalosporinase activities of the protein. The beta-lactamase has been purified to homogeneity and found to bind [3H]benzylpenicillin, a result reflecting a rate-limiting deacylation step. Nucleotide sequences homologous to bla were found in all tested cephamycin producers, but several other Streptomyces species which produce a beta-lactamase do not contain genes for beta-lactam antibiotic biosynthesis.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Perez-Llarena, F.

Martin, Juan F.

Galleni, Moreno ; Université de Liège - ULiège

Coque, J. J.

Fuente, J. L.

Frère, Jean-Marie ; Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines

Liras, P.

Language :

English

Title :

The bla gene of the cephamycin cluster of Streptomyces clavuligerus encodes a class A beta-lactamase of low enzymatic activity.

Publication date :

1997

Journal title :

Journal of Bacteriology

ISSN :

0021-9193

eISSN :

1098-5530

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Volume :

179

Issue :

Pages :

6035-40

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 01 December 2015

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