Article (Scientific journals)
The bla gene of the cephamycin cluster of Streptomyces clavuligerus encodes a class A beta-lactamase of low enzymatic activity.
Perez-Llarena, F.; Martin, Juan F.; Galleni, Moreno et al.
1997In Journal of Bacteriology, 179 (19), p. 6035-40
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Keywords :
Amino Acid Sequence; Anti-Bacterial Agents/biosynthesis/metabolism; Base Sequence; Cephamycins/biosynthesis; Cloning, Molecular; Genes, Bacterial; Kinetics; Molecular Sequence Data; Molecular Weight; Penicillin G/metabolism; Recombinant Proteins/metabolism; Streptomyces/enzymology/genetics; Transformation, Genetic; beta-Lactamases/chemistry/genetics/metabolism
Abstract :
[en] A gene (bla) encoding a beta-lactamase is present in the cephamycin gene cluster of Streptomyces clavuligerus, the strain producing clavulanic acid and a beta-lactamase inhibitory protein. The bla gene is located 5.1 kb downstream from and in the opposite orientation to cefE, encoding the deacetoxycephalosporin C synthase. The bla gene encodes a 332-residue protein (Mr, 35,218), similar to other class A beta-lactamases produced by actinomycetes. Modification (to SDG) of the SDN conserved motif of class A beta-lactamases as well as of amino acids in otherwise conserved regions in the molecule may explain the low penicillinase and cephalosporinase activities of the protein. The beta-lactamase has been purified to homogeneity and found to bind [3H]benzylpenicillin, a result reflecting a rate-limiting deacylation step. Nucleotide sequences homologous to bla were found in all tested cephamycin producers, but several other Streptomyces species which produce a beta-lactamase do not contain genes for beta-lactam antibiotic biosynthesis.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Perez-Llarena, F.
Martin, Juan F.
Galleni, Moreno ;  Université de Liège - ULiège
Coque, J. J.
Fuente, J. L.
Frère, Jean-Marie ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Liras, P.
Language :
English
Title :
The bla gene of the cephamycin cluster of Streptomyces clavuligerus encodes a class A beta-lactamase of low enzymatic activity.
Publication date :
1997
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
179
Issue :
19
Pages :
6035-40
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 December 2015

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