[en] The beta-lactamase of Mycobacterium smegmatis mc(2)155 has been purified to protein homogeneity. Its N-terminal sequence and catalytic properties are similar to those of the beta-lactamase produced by Mycobacterium fortuitum D316 and establish this new enzyme as a member of molecular class A.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Quinting, B.
Galleni, Moreno ; Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Timm, J.
Gicquel, B.
Amicosante, G.
Frère, Jean-Marie ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Language :
English
Title :
Purification and Properties of the Mycobacterium Smegmatis Mc(2)155 Beta-Lactamase
Jarlier, V. and Nikaido, H. (1990) J. Bacteriol. 172, 1418-1423.
Zhang, Y., Heyen, B., Allen, B., Young, D. and Cole, S. (1992) Nature 358, 591.
Jarlier, V., Gutmann, L. and Nikaido, H. (1991) Antimicrob. Agents Chemother. 35, 1937-1939.
Finck, R. (1986) J. Antimicrob. Chemother. 18, 6-8.
Zhang, Y., Wallace, J.R., Steingrube, V.A., Brown, B.A., Nash, R., Silox, A. and Tsukamura, M. (1992) Tubercule Lung Dis. 73, 337-344.
Amicosante, G., Franceschini, N., Segatore, B., Oratore, A., Fattorini, L., Orefici, G., Van Beeumen, J. and Frère, J.M. (1990) Biochem. J. 271, 729-734.
Timm, J., Perilli, M.G., Duez, C., Trias, J., Orefici, G., Fattorini, L., Oratore, A., Joris, B., Frère, J.M., Pugsley, A.P. and Gicquel, B. (1994) Mol. Microbiol. 12, 491-504.
Fattorini, L., Scorideci, G., Jin, S.H., Amicosante, G., Franceschini, N., Oratore, A. and Orefici, G. (1991) Antimicrob. Agents Chemother. 85, 1760-1764.
Dubus, A., Monnaie, D., Jacobs, C., Normark, S. and Frère, J.M. (1993) Biochem. J. 292, 537-543.
Snapper, S.B., Melton, R.E., Kieser, T. and Jacobs, W.R. (1990) Mol. Microbiol. 11, 1911-1919.
Bradford, M.M. (1976) Anal. Biochem. 72, 248-254.
Laemmli, U.K. (1970) Nature 227, 680-685.
Joris, B., De Meester, F., Galleni, M., Reckinger, G., Coyette, J., Frère, J.M. and Van Beeumen, J. (1985) Biochem. J. 221, 505-512.
De Meester, F., Joris, B., Reckinger, G., Bellefroid-Bourguignon, C., Frère, J.M. and Waley, S.G. (1987) Biochem. Pharmacol. 36, 2393-2403.
Ambler, R.P., Coulson, A.F., Frère, J.M., Ghuysen, J.M., Jaurin, B., Joris, B., Levesque, R., Tiraby, G. and Waley, S.G. (1991) Biochem. J. 276, 269-272.
