Comparison of serum fractionation methods by data independent label-free proteomics

Baiwir, Dominique; Mazzucchelli, Gabriel; Smargiasso, Nicolas; QUESADA CALVO, Florence; De Pauw, Edwin; MALAISE, Michel; Louis, Edouard; MEUWIS, Marie-Alice

doi:10.1016/j.euprot.2015.07.009

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Comparison of serum fractionation methods by data independent label-free proteomics

Baiwir, Dominique; Mazzucchelli, Gabriel; Smargiasso, Nicolas et al.

2015 • In EuPA Open Proteomics, 9, p. 14-22

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/185851

DOI
10.1016/j.euprot.2015.07.009

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Keywords :

Serum prefractionnation; Repeatability; Label-free Proteomics; Data Independent; DEpletion; Equalization

Abstract :

[en] Off-line sample prefractionations applied prior to biomarker discovery proteomics are options to enable more protein identifications and detect low-abundance proteins. This work compared five commercial methods efficiency to raw serum analysis using label-free proteomics. The variability of the protein quantities determined for each process was similar to the unprefractionated serum. A 49% increase in protein identifications and 12.2% of reliable quantification were obtained. A 61 times lower limit of protein quantitation was reached compared to protein concentrations observed in raw serum. The concentrations of detected proteins were confronted to estimated reference values.

Disciplines :

Human health sciences: Multidisciplinary, general & others
Chemistry
Biochemistry, biophysics & molecular biology

Author, co-author :

Baiwir, Dominique ; Université de Liège > GIGA-Management : Plate-forme protéomique

Mazzucchelli, Gabriel ; Université de Liège > Center for Analytical Research and Technology (CART)

Smargiasso, Nicolas ; Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

QUESADA CALVO, Florence ; Centre Hospitalier Universitaire de Liège - CHU > Gastro-Entérologie-Hépatologie

De Pauw, Edwin ; Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

MALAISE, Michel ; Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie

Louis, Edouard ; Université de Liège > Département des sciences cliniques > Hépato-gastroentérologie

MEUWIS, Marie-Alice ; Centre Hospitalier Universitaire de Liège - CHU > Gastro-Entérologie-Hépatologie

Language :

English

Title :

Comparison of serum fractionation methods by data independent label-free proteomics

Publication date :

December 2015

Journal title :

EuPA Open Proteomics

eISSN :

2212-9685

Publisher :

Elsevier

Volume :

Pages :

14-22

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://dx.doi.org/10.1016/j.euprot.2015.07.009

Available on ORBi :

since 12 September 2015

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