[en] Habituated sugar beet calli have been characterized as having a deficiency in some tetrapyrrole containing compounds. However, peroxidases might be dissociated from the other tetrapyrrole containing compounds. When light-cultured normal and habituated calli were transferred to darkness their peroxidase activity reduced and increased, respectively, indicating that habituation could not strictly be characterized by a deficiency in peroxidase content but rather by a different regulation of its activity. This regulation could be mediated through soluble effectors which act as potential peroxidase inhibitors and/or by a differential expression of the peroxidase isoenzyme patterns which were present in these tissues in both light and darkness. The different peroxidase activity and the nature of acidic and basic isoenzymes in normal and habituated tissues could explain the different features of both types of cultures.
Bernal, M.A., Pedreño, M.A., Calderón, A.A., Muñńoz, R., Ros Barceló, A., Merino de Cáceres, F.: The subcellular localization of isoperoxidases in Capsicum annuum leaves and their different expression in vegetative and flowered plants. - Ann. Bot. 72: 415-421, 1993.
Bernal, M.A., Calderón, A.A., Pedreño, M.A., Ferrer, M.A., Merino de Cáceres, F., Ros Barceló, A.: Purification of a basic peroxidase isoenzyme from Capsicum fruits and the immunoinhibition of its capsaicin oxidation capacity by antibodies raised against horseradish peroxidase. - Z. Lebensm. Unter. Forsch. 199: 240-242, 1994.
Bernal, M.A., Calderón, A.A., Ferrer, M.A., Merino de Cáceres, F., Ros Barceló, A.: Oxidation of capsaicin and capsaicin phenolic precursors by the basic peroxidase isoenzyme B6 from hot pepper. - J. Agr. Food Chem. 43: 352-355, 1995.
Bisbis, B., Dujardin, E., Kevers, C., Hagège, D., Gaspar, T.: Chlorophylls and carotenoids in a fully nonorganogenic callus of Beta vulgaris. - Biol. Plant. 36: 443-449, 1994.
Blom, T.J.M., Sierra, M., van Vliet, T.B., Franke-van Dijk, M.E.I., de Koning, P., van Iren, F., Verpoorte, R., Libbenga, K.R.: Uptake and accumulation of ajmalicine into isolated vacuoles of cultured cells of Catharanthus roseus (L.) G. Don. and its conversion into serpentine. - Planta 183: 170-177, 1991.
Crèvecoeur, M., Kevers, C., Greppin, H., Gaspar, T.: A comparative biochemical and cytological characterization of normal and habituated sugar beet calli. - Biol. Plant. 29: 1-6, 1987.
Engelmann, I., Macheix, J., Caspar, T.: Phenolic compounds in hormone dependent and independent sugar beet callus lines compared to donor plants. - Saussurea 24: 15-21, 1993.
Ferrer, M.A., Calderón, A.A., Muñoz, R., Ros Barceló, A.: 4-Methoxy-α-naphthol as a specific substrate for kinetic, zymographic and cytochemical studies on plant peroxidase activities. - Phytochem. Anal. 1: 63-69, 1990.
Ferrer, M.A., Pedreño, M.A., Muñoz, R., Ros Barceló, A.: Soluble peroxidase gradients in lupin hypocotyls and the control of the level of polarly transported indole-3yl-acetic acid. - J. Plant Growth Regul. 10: 139-146, 1991.
Ferrer, M.A., Pedreño, M.A., Ros Barceló, A., Muñoz, R.: The cell wall localization of two strongly basic isoperoxidases in etiolated Lupinus albus hypocotyls and its significance in coniferyl alcohol oxidation and indole-3-acetic acid catabolism. - J. Plant Physiol. 139: 611-616, 1992.
Gaspar, T., Penel, C., Thorpe, T., Greppin, H.: Peroxidases 1970-1980. A Survey of their Biochemical and Physiological Roles in Higher Plants. - Univ. Geneva, Centre de Botanique Publ., Geneva 1982.
Hagège, D., Penel, C., Crèvecoeur, M., Kevers, C., Gaspar, T., Boucaud, J., Greppin, H.: Plasmamembrane peroxidases from the auxin- and cytokinin-independent sugar beet callus. - Arch. int. Physiol. Biochim. Biophys. 99: 2, 1991a.
Hagège, D., Kevers, C., Crèvecoeur, M., Tollier, M.T., Monties, B., Gaspar, T.: Peroxidases, growth and differentiation of habituated sugar beet cells. - In: Lobarszewski, J., Greppin, H., Penel, C., Gaspar, T. (ed.): Biochemical, Molecular and Physiological Aspects of Plant Peroxidases. Pp. 281-290. University of Lublin, Lublin and University of Geneva, Geneva 1991b.
Hagège, D., Werck-Reichhart, D., Schmitt, P., Gaspar, T.: Deficiency in tetrapyrrole containing compounds in a non-organogenic habituated sugar beet cell line. - Plant Physiol. Biochem. 30: 649-654, 1992a.
Hagège, D., Kevers, C., Salabert, P., Gaspar, T.: Protective systems against activated oxygen species compared in normal and fully habituated nonorganogenic sugar beet calluses. - In Vitro Cell Dev. Biol. 28: 143-147, 1992b.
Kamínek, M., Hadačová, V., Luštinec, J.: Plastids and the origin of cytokinin autonomy in tobacco tissue cultures. - In: Guern, J., Péaud-Le Noël, C. (ed.): Metabolism and Molecular Activities of Cytokinins. Pp. 242-251. Springer-Verlag, Berlin 1981.
Kevers, C., Coumans, M., De Greef, W., Hofinger, M., Gaspar, T.: Habituation in sugar beet callus: auxin content, auxin protectors, peroxidase pattern and inhibitors. - Physiol. Plant. 51: 281-286, 1981.
Kevers, C., Bisbis, B., Le Dily, F., Billard, J.P., Huault, C., Gaspar, T.: Darkness improves growth and delays necrosis in a nonchlorophyllous habituated sugar beet callus: biochemical changes. - In Vitro Cell Dev. Biol. 31: 122-126, 1995.
Krsnik-Rasol, M.: Peroxidase as a developmental marker in plant tissue culture. - Int. J. Dev. Biol. 35: 259-263, 1991.
Le Dily, F., Billard, J.P., Gaspar, T., Huault, C.: Disturbed nitrogen metabolism associated with the hyperhydric status of fully habituated callus of sugar beet. - Physiol. Plant. 88: 129-134, 1993.
Liners, F., Gaspar, T., van Cutsem, P.: Acetyl- and methyl-esterification of pectins of friable and compact sugar beet calli: consequences for intercellular adhesion. - Planta 192: 545-556, 1994.
Penel, C., Sticher, L., Kevers, C., Gaspar, T., Greppin, H.: Calcium-controlled peroxidase secretion by sugar beet cells: effect of ionophores in relation to organogenesis. - Biochem. Physiol. Pflanz. 179: 173-180, 1984.
Penel, C., Bernardini, N., Greppin, H.: Production and characterization of monoclonal antibodies to a cationic peroxidase from spinach leaves. - Plant Sci. 67: 7-19, 1990.
Perrey, R., Hauser, M.T., Wink, M.: Cellular and subcellular localization of peroxidase isoenzymes in plants and cell suspension cultures from Lupinus polyphyllus. - Z. Naturforsch. 44c: 931-936, 1989.
Ros Barceló, A., Muñoz, R., Sabater, F.: Lupin peroxidases. I. Isolation and characterization of cell wall-bound isoperoxidase activity. - Physiol. Plant. 71: 448-454, 1987.
Ros Barceló, A., Muñoz, R., Sabater, F.: Subcellular location of basic and acidic soluble isoperoxidases in Lupinus. - Plant Sci. 63: 31-38, 1989.
Ros Barceló, A., Pedreño, M.A., Ferrer, M.A., Sabater, F., Muñoz, R.: Indole-3-methanol is the main product of the oxidation of indole-3-acetic acid catalyzed by two cytosolic basic isoperoxidases from Lupinus. - Planta 181: 448-450, 1990.
