Reference : Dual-specificity phosphatase 3 knockout female mice are resistant to LPS and to polym...
Scientific congresses and symposiums : Paper published in a journal
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/181118
Dual-specificity phosphatase 3 knockout female mice are resistant to LPS and to polymicrobial induced septic shock in TNF dependent manner.
English
Rahmouni, Souad mailto [Université de Liège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Singh []
Dejager, Lien []
Singh, Maneesh []
Amand, Mathieu mailto [Université de Liège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Musumeci, Lucia mailto [Université de Liège > > GIGA-Research >]
Moutschen, Michel mailto [Université de Liège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Mustelin, Tomas []
Libert, Claude []
May-2013
Journal of Immunology
American Association of Immunologists
Yes (verified by ORBi)
No
International
0022-1767
1550-6606
Baltimore
MD
Immunology 2013 - Annual Meeting of the AAI
du 03 au 07 Mai 2013
AAI
Honolulu
USA
[en] We report the generation of dual-specificity phosphatase 3 (DUSP3) deficient mice. These mice develop normally and do not exhibit any spontaneous phenotype. However, VHR-/- females, but not males, are resistant to LPS- and to polymicrobial infection-induced septic shock. After LPS injection, while VHR-/- males and VHR+/+ mice of both genders, displayed an increased serum levels of TNF-α and IFN, the levels of these cytokines remained significantly low in the VHR-/- females. In vitro experiments using peritoneal macrophages showed the same results suggesting that the systemic cytokines profiles observed are macrophages-dependent. Adoptive transfer of VHR-/- females bone marrow to irradiated VHR+/+ female mice, but not to VHR-/- or VHR+/+ males, protected them from death after administration of LPS. Interestingly, VHR-/- females were sensitive to TNF-α- induced lethality. We also report that the decrease of TNF-α production observed in VHR-/- female’s macrophages after LPS activation was associated with a decreased ERK1/2, but not MEK1/2, activation. Interestingly, pervanadate (PTP pan inhibitor) treatment prior to LPS activation restored ERK1/2 activation in the VHR-deficient macrophages, suggesting that VHR is targeting one of the ERK1/2 PTPs or DUSPs. These results, together with our observation that DUSP3 is the most highly expressed phosphatase in macrophages, suggest a key non-redundant role of VHR as positive regulator of TNF-α in innate immune response in females.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/181118

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Dual-specificity phosphatase 3 knockout female mice are resistant to LPS and to polymicrobial induced septic shock in TNF dependent manner. (P1222) -- Rahmouni et al. 190 (1001): 138.9 -- The Journal of Immunology.pdfPublisher postprint86.05 kBView/Open

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