Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia
Callus induction; Leaf-derived calli; Hawthorn; Chemical analysis; Anrtiradical activities
Abstract :
[en] The present research work describes the effects of 2,4-dichlorophenoxyacetic acid (2,4-D)/6-Benzylamino-purine (BAP) ratio on callus induction, total phenols and ascorbic acid production and antioxidant activities in leaf-derived calli of Crataegus azarolus (hawthorn). The supplementation of 1.0 mg/L 2,4-D and 1.0 mg/L BAP to MS medium was found to be the most efficient for callus induction (as percentage and fresh weigh). The results of biochemical analysis showed that the highest total phenols contents were obtained in callus cultured on MS medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP (52 ± 0.56 mg GAE/g DM) and were significantly lower than those of intact leaves (76 ± 1.72 mg GAE/g DM). However, the highest ascorbic acid contents were found in callus cultured on MS supplemented with 1.0 mg/L 2,4-D and 0.5 mg/L BAP (0.96 ± 0.13 mg AAE/g DM) and these amounts were statistically similar to those found in leaves tissues (0.74 ± 0.07 mg AAE/g DM). Antioxidant activities of callus extracts were determined using two TEAC assays and results showed that extract of callus cultured on MS medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP have the greatest antiradical activities against DPPH (124 ± 2.92 mg TE/g DM) and ABTS (0.19 ± 0.02 mg TE/g DM) compared to the leaves of field-grown plant. Thus, the use of high level of 2,4-D over BAP can be suitable to enhance the quality more than the quantity of bioactive compounds in leaf callus culture of hawthorn.
Chaabani, Ghaya; University of Tunis > Physiology and Biochemistry
Tabart, Jessica ; Université de Liège - ULiège > PLant Molecular Biology and Biotechnology
Kevers, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Dommes, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Khan, Muhammad
Zaoui, Sonia
Chebchoud, Lotfi
Lachaal, Mokhtar
Karray-Bouraoui, Najoua
Language :
English
Title :
Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia
Al Abdallat AM, Sawwan JS, Al Zoubi B (2011) Agrobacterium tumefaciens-mediated transformation of callus cells of Crataegus aronia. Plant Cell Tiss Organ Cult 104:31–39. doi:10.1007/s11240-010-9798-1
Association of vitamin chemists (1961) Methods of vitamin assay, 3rd edn. Interscience Publishers, New York
Bahorun T (1995) Les polyphénols de Crataegus monogyna Jacq. in vivo et in vitro: analyses et activités antioxydantes. Dissertation, Université de Lille I
Bahorun T, Trotin F, Pommery J, Vasseur J, Pinkas M (1994) Antioxidant activities of Crataegus monogyna extracts. Planta Med 60(4):323–328
Bahorun T, Aumjaud E, Ramphul H, Rycha M, Luximon-Ramma A, Trotin F, Aruoma OI (2003) Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts. Nahrung 47(3):191–198. doi:10.1002/food.200390045
Bahri-Sahloul R, Ben Fredj R, Boughalleb N, Shriaa J, Saguem S, Hilbert JL, Trotin F, Ammar S, Bouzid S, Harzallah-Skhiri F (2014) Phenolic composition and antioxidant and antimicrobial activities of extracts obtained from Crataegus azarolus L. var. aronia (Willd.) Batt. ovaries calli. J Bot 2014:1–11. doi:10.1155/2014/623651
Bignami C, Paolocci M, Scossa A (2003) Preliminary evaluation of nutritional and medicinal components of Crataegus azarolus fruits. Acta Hortic 597:95–100
Bor Z, Arslan R, Bektas N, Pirildar S, Donmez AA (2012) Antinociceptive, antiinflammatory, and antioxidant activities of the ethanol extract of Crataegus orientalis leaves. Turk J Med Sci 42(2):315–324. doi:10.3906/sag-1011-1304
Caboni E, Tonelli MG, Lauri P, Iacovacci P, Kevers C, Damiano C, Gaspar T (1997) Biochemical aspects of almond microcuttings related to in vitro rooting ability. Biol Plantarum 39:91–97
Chong TM, Abdullah MA, Fadzillah NM, Lai OM, Lajis NH (2004) Anthraquinones production, hydrogen peroxide level and antioxidant vitamins in Morinda elliptica cell suspension cultures from intermediary and production medium strategies. Plant Cell Rep 22(12):951–958
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N (2006) Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem 97:654–660. doi:10.1016/j.foodchem.2005.04.028
Dou D, Leng P, Li Y, Zeng Y, Sun Y (2013) Comparative study of antioxidant compounds and antiradical properties of the fruit extracts from three varieties of Crataegus pinnatifida. J Food Sci Technol. doi:10.1007/s13197-013-0954-6
Duangporn P, Siripong P (2009) Effect of auxin and cytokinin on phyllanthusol A production by callus cultures of Phyllanthus acidus skeels. Am Eurasia J Agric Environ Sci 5:258–263
El-Baz FK, Mohamed AA, Ali SI (2010) Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrulluscolosynthis). Nova biotechnol 10(2):79–94
Grzegorczyk I, Matkowski A, Wysokinska H (2007) Antioxidant activity of extracts from in vitro cultures of Salvia officinalis L. Food Chem 104:536–541. doi:10.1016/j.foodchem.2006.12.003
Kartnig T, Kogl G, Heydel B (1993) Production of flavonoids in cell cultures of Crataegus monogyna. Planta Med 59(6):537–538
Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY (2003) Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agr Food Chem 51:6509–6515. doi:10.1021/jf0343074
Lakshmi T, Geetha RV, Anitha R (2012) Crataegus oxyacantha Linn commonly known as Hawthorn—a scientific review. Int J PharmTech Res 4(1):458–465
Loewus FA, Kelly S (1991) Identity of l-ascorbic acid formed from d-glucose by strawberry (Fragaria). Nature 191:1059–1061. doi:10.1038/1911059a0
Maharik N, Elgengaihi S, Taha H (2009) Anthocyanin production in callus cultures of Crataegus sinaica boiss. Int J Acad Res 1:30–34
Matkowski A (2008) Plant in vitro culture for the production of antioxidants—a review. Biotechnol Adv 26(6):548–560. doi:10.1016/j.biotechadv.2008.07.001
Mori T, Sakurai M, Seki M, Furusaki S (1994) Use of axin and cytokinin to regulate anthocyanin production and composition in suspension cultures of strawberry cell. J Sci Food Agric 65:271–276
Murashige M, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497
Nag R, Chaturvedi A, Sharma A, Bidawat S, Pareek LK, Nag TN (2012) Estimation of ascorbic acid content in Prosopis (L.) Druce Cultivar K1. Plant Tissue Cult Biotech 22(1):27–32
Norhayati Y, Nor `Aini MF, Misri K, Marziah M, Azman J (2011) α-tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissues and callus cultures. Pertanika J Trop Agric Sci 34(2):331–339
Rakotoarison DA, Gressier B, Trotin F, Brunet C, Dine T, Luyckx M, Vasseur J, Cazin M, Cazin JC, Pinkas M (1997) Antioxidant activities of polyphenolic extracts from flowers, in vitro callus and cell suspension cultures of Crataegus monogyna. Pharmazie 52:60–64
Ramachandra Rao S, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20(2):101–153. doi:10.1016/S0734-9750(02)00007-1
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26:1231–1237. doi:10.1016/S0891-5849(98)00315-3
Simoés C, Brasil Bizarri CH, Da Silva Cordeiro L, Carvalho de Castro T, Machado Coutada LC, Ribeiro da Silva AJ, Albarello N, Mansur E (2009) Anthocyanin production in callus cultures of Cleome rosea: modulation by culture conditions and characterization of pigments by means of HPLC-DAD/ESIMS. Plant Physiol Biochem 47(10):895–903. doi:10.1016/j.plaphy.2009.06.005
Singh V, Nag TN (1991) Production of endogenous ascorbic acid from tissue cultures of Abutilon pannosum Forst. J Phytol Res 4:13–19
Tabart J, Kevers C, Sipel A, Pincemail J, Defraigne JO, Dommes J (2006) Antioxidant capacity of black currant varies with organ, season, and cultivar. J Agric Food Chem 54:6271–6276. doi:10.1021/jf061112y
Tabart J, Kevers C, Sipel A, Pincemail Defraigne JO, Dommes J (2007) Optimisation of extraction of phenolics and antioxidants from black currant leaves and buds and of stability during storage. Food Chem 105:1268–1275
Tadolini B, Juliano C, Piu L, Franconi F, Cabrini L (2000) Resveratrol inhibition of lipid peroxidation. Free Radical Res 33:105–114. doi:10.1080/10715760000300661
Taha HS, Rahman AE, Fathalla RA, Kareem MA, Aly UE (2008) Successful application for enhancement and production of anthocyantn pigment from calli cultures of some ornamental plants. Aust J Basic Appl 2(4):1148–1156
Tahirović A, Čopra–Janićijević A, Bašić N, Klepo L, Subašić M (2012) Determination of vitamin C in flowers of some bosnian Crataegus L. species. Works of the Faculty of Forestry University of Sarajevo 42(2): 1-12
Toker G, Memisoglu M, Toker MC, Yesilada E (2003) Callus formation and cucurbitacin B accumulation in Echallium elaterium callus cultures. Fitoterapia 74:618–623
Verma SK, Jain V, Verma D, Khamesra R (2007) Crataegus oxyacantha—a cardioprotective herb. J Herb Med Toxicol 1:65–71
