Development of recombinant stable house dust mite allergen Der p 3 molecules for component-resolved diagnosis and specific immunotherapy.

Bouaziz, Ahlem; Walgraffe, David; Bouillot, Celine; Herman, Julie; FOGUENNE, Jacques; Gothot, André; Louis, Renaud; Hentges, Francois; Jacquet, Alain; Mailleux, Anne-Catherine; Chevigne, Andy; Galleni, Moreno; Adam, Emmanuelle; Dumez, Marie-Eve

doi:10.1111/cea.12452

Article (Scientific journals)

Development of recombinant stable house dust mite allergen Der p 3 molecules for component-resolved diagnosis and specific immunotherapy.

Bouaziz, Ahlem; Walgraffe, David; Bouillot, Celine et al.

2015 • In Clinical and Experimental Allergy, 45

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/176927

DOI
10.1111/cea.12452

PubMed
25406386

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Keywords :

Der p 3; diagnosis; hypoallergen; inactive enzyme; recombinant mite allergen; specific immunotherapy

Abstract :

[en] BACKGROUND: The allergen Der p 3 is underrepresented in house dust mite (HDM) extracts probably due to autolysis. Recombinant stable molecule of the allergen is thus needed to improve the diagnosis of allergy and the safety and efficacy of immunotherapy. OBJECTIVE: The current study reports the immunological characterization of two recombinant molecules of the HDM allergen Der p 3 as useful tools for diagnosis and immunotherapy. METHODS: Recombinant mature (rDer p 3) and immature (proDer p 3) Der p 3 and their corresponding S196A mutants were produced in Pichia pastoris and purified. The stability, IgE-binding capacity and allergenicity of the different proteins were analyzed and compared with those of the major mite allergen Der p 1 used as a reference. Additionally, the immunogenicity of the different allergens was evaluated in a murine model of Der p 3 sensitization. RESULTS: Compared to the IgE reactivity to recombinant and natural Der p 3 (nDer p 3), the mean IgE binding of patient's sera to rDer p 3-S196A (50 %) was higher. The poorly binding to nDer p 3 or rDer p 3 was due to autolysis of the allergen. Contrary to Der p 3, proDer p 3 displayed very weak IgE reactivity, as measured by sandwich ELISA and competitive inhibition, RBL degranulation and human basophil activation assays. Moreover, proDer p 3 induced a TH 1-biased immune response that prevented an allergic response in mice but retained Der p 3-specific T cell reactivity. CONCLUSION: rDer p 3-S196A should be used for the diagnosis of HDM allergy elicited by Der p 3, and proDer p 3 may represent a hypoallergen of Der p 3. This article is protected by copyright. All rights reserved.

Research Center/Unit :

CIP - Centre d'Ingénierie des Protéines - ULiège

Disciplines :

Immunology & infectious disease

Author, co-author :

Bouaziz, Ahlem ; Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines

Walgraffe, David

Bouillot, Celine

Herman, Julie

FOGUENNE, Jacques ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie

Gothot, André ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la coagulation et de l'hémostase

Louis, Renaud ; Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie

Hentges, Francois

Jacquet, Alain

Mailleux, Anne-Catherine

More authors (4 more)

Language :

English

Title :

Development of recombinant stable house dust mite allergen Der p 3 molecules for component-resolved diagnosis and specific immunotherapy.

Publication date :

2015

Journal title :

Clinical and Experimental Allergy

ISSN :

0954-7894

eISSN :

1365-2222

Publisher :

Blackwell, Oxford, United Kingdom

Volume :

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

Mise au point d’une forme stable de l’allergène Der p 3 de Dermatophagoides pteronyssinus pour le diagnostic rapide et le développement de nouvelles approches d’immunothérapie de l’allergie aux acariens

Funders :

ULiège - Université de Liège

Commentary :

Available on ORBi :

since 20 January 2015

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