One-pot enzymatic synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc sequence with in situ UDP-Gal regeneration.

Animals; Carbohydrate Sequence; Cattle; Enzyme Stability; Galactosyltransferases/chemistry; Glucosyltransferases/chemistry; Kinetics; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Oligosaccharides/chemical synthesis; UDPglucose 4-Epimerase/chemistry; Uridine Diphosphate Galactose/chemistry

Abstract :

[en] The trisaccharide Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was enzymatically synthesized, with in situ UDP-Gal regeneration. By combination in one pot of only four enzymes, namely, sucrose synthase, UDP-Glc 4'-epimerase, UDP-Gal:GlcNAc beta 4-galactosyltransferase and UDP-Gal:Gal beta 1-->4GlcNAc alpha 3-galactosyltransferase, Gal alpha 1-->3Gal beta 1-->4GlcNAc beta 1-->O-(CH2)8COOCH3 was formed in a 2.2 mumol ml-1 yield starting from the acceptor GlcNAc beta 1-->O-(CH2)8COOCH3. This is an efficient and convenient method for the synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc epitope which pays an important role in various biological and immunological processes.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Hokke, Cornelis H

Zervosen, Astrid ; Université de Liège - ULiège > Centre de recherches du cyclotron

Elling, Lothar

Joziasse, David H.

van den Eijnden, Dirk H.

Language :

English

Title :

One-pot enzymatic synthesis of the Gal alpha 1-->3Gal beta 1-->4GlcNAc sequence with in situ UDP-Gal regeneration.

Publication date :

1996

Journal title :

Glycoconjugate Journal

ISSN :

0282-0080

eISSN :

1573-4986

Publisher :

Kluwer Academic Publishers, Boston, United States - Massachusetts

Volume :

Issue :

Pages :

687-92

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 July 2009

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