Combined enzymatic synthesis of nucleotide (deoxy) sugars from sucrose and nucleoside monophosphates

[en] Abstract: The synthesis of NDP-glucose 3a-d (N= A, C, U, dU) with sucrose synthase B was combined with the enzymatic synthesis of nucleoside diphosphates 2a-d from their corresponding nucleoside monophosphates la-d by different kinases A. Further combination with recombinant dTDP-glucose 4,6-dehydratase D enabled us to synthesize dUDP-6-deoxy-alpha-D-xylo-4-hexulose 5 from ld on a preparative scale. By using the repetitive batch technique the enzymatic syntheses of nucleotide (deoxy) sugars 3a-d, 5 could be realized on a 0.1 - 0.5 g scale.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Zervosen, Astrid ; Université de Liège - ULiège > Centre de recherches du cyclotron

Stein, Andreas

Adrian, Holger

Elling, Lothar

Language :

English

Title :

Combined enzymatic synthesis of nucleotide (deoxy) sugars from sucrose and nucleoside monophosphates

Publication date :

1996

Journal title :

Tetrahedron

ISSN :

0040-4020

Publisher :

Pergamon Press, Oxford, United Kingdom

Volume :

Issue :

Pages :

2395-2404

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 July 2009

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