Production of a novel mixture of mycosubtilins by mutants of Bacillus subtilis.

Antifungal Agents; Bacillus subtilis/genetics/metabolism; Bioreactors; Biotechnology/methods; Chromatography, High Pressure Liquid; Escherichia coli; Gene Knockout Techniques; Lipopeptides/genetics; Lipoproteins/biosynthesis; Peptides, Cyclic/genetics; Plasmids/genetics; Protein Isoforms/biosynthesis; Species Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry; Temperature; Antifungal activity; Bacillus subtilis; Mycosubtilin; Novel isoforms; Spreading

Abstract :

[en] Using promoter exchange and gene knock-out strategies, two mutant strains, the so-called BBG116 and BBG125, were constructed from Bacillus subtilis wild-type strain ATCC 6633, a surfactin and mycosubtilin producer. Compared to the parental strain, both mutants overproduced constitutively mycosubtilin, while BBG125 had lost the ability to synthesize surfactin. Surprisingly, BBG125 was found to produce about 2-fold less mycosubtilin than BBG116 despite an expected higher availability of the cytoplasmic precursors and cofactors pool for biosynthesis. Further physiological characterization of BBG125 also highlighted: (i) a strong influence of temperature on mycosubtilin biosynthesis in BBG125 with a maximal productivity observed at 22 degrees C, compared to 15 and 30 degrees C; (ii) substantial changes in fatty acid profiles and thereby in mycosubtilin isoforms, compared to the wild-type strain; and (iii) the presence of five novel mycosubtilin isoforms. The antifungal activities of the new mix were higher than or equal to those of purified isoforms.

Disciplines :

Microbiology

Author, co-author :

Bechet, Max

Castera-Guy, Joany

Guez, Jean-Sebastien

Chihib, Nour-Eddine

Coucheney, Francoise

Coutte, Francois

Fickers, Patrick ; Université Libre de Bruxelles - ULB > Biotechnologies et Bioprocedes

Leclere, Valerie

Wathelet, Bernard ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle

Jacques, Philippe ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries

Language :

English

Title :

Production of a novel mixture of mycosubtilins by mutants of Bacillus subtilis.

Publication date :

2013

Journal title :

Bioresource Technology

ISSN :

0960-8524

eISSN :

1873-2976

Publisher :

Elsevier, Netherlands

Volume :

145

Pages :

264-70

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 21 October 2014

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