Article (Scientific journals)
Production of a novel mixture of mycosubtilins by mutants of Bacillus subtilis.
Bechet, Max; Castera-Guy, Joany; Guez, Jean-Sebastien et al.
2013In Bioresource Technology, 145, p. 264-70
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Keywords :
Antifungal Agents; Bacillus subtilis/genetics/metabolism; Bioreactors; Biotechnology/methods; Chromatography, High Pressure Liquid; Escherichia coli; Gene Knockout Techniques; Lipopeptides/genetics; Lipoproteins/biosynthesis; Peptides, Cyclic/genetics; Plasmids/genetics; Protein Isoforms/biosynthesis; Species Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry; Temperature; Antifungal activity; Bacillus subtilis; Mycosubtilin; Novel isoforms; Spreading
Abstract :
[en] Using promoter exchange and gene knock-out strategies, two mutant strains, the so-called BBG116 and BBG125, were constructed from Bacillus subtilis wild-type strain ATCC 6633, a surfactin and mycosubtilin producer. Compared to the parental strain, both mutants overproduced constitutively mycosubtilin, while BBG125 had lost the ability to synthesize surfactin. Surprisingly, BBG125 was found to produce about 2-fold less mycosubtilin than BBG116 despite an expected higher availability of the cytoplasmic precursors and cofactors pool for biosynthesis. Further physiological characterization of BBG125 also highlighted: (i) a strong influence of temperature on mycosubtilin biosynthesis in BBG125 with a maximal productivity observed at 22 degrees C, compared to 15 and 30 degrees C; (ii) substantial changes in fatty acid profiles and thereby in mycosubtilin isoforms, compared to the wild-type strain; and (iii) the presence of five novel mycosubtilin isoforms. The antifungal activities of the new mix were higher than or equal to those of purified isoforms.
Disciplines :
Microbiology
Author, co-author :
Bechet, Max
Castera-Guy, Joany
Guez, Jean-Sebastien
Chihib, Nour-Eddine
Coucheney, Francoise
Coutte, Francois
Fickers, Patrick ;  Université Libre de Bruxelles - ULB > Biotechnologies et Bioprocedes
Leclere, Valerie
Wathelet, Bernard ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Jacques, Philippe ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Language :
English
Title :
Production of a novel mixture of mycosubtilins by mutants of Bacillus subtilis.
Publication date :
2013
Journal title :
Bioresource Technology
ISSN :
0960-8524
eISSN :
1873-2976
Publisher :
Elsevier, Netherlands
Volume :
145
Pages :
264-70
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2013 Elsevier Ltd. All rights reserved.
Available on ORBi :
since 21 October 2014

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