Reference : Multidisciplinary approaches and fractionations to study lower termite symbiotic syst...
Scientific congresses and symposiums : Poster
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Entomology & pest control
http://hdl.handle.net/2268/173197
Multidisciplinary approaches and fractionations to study lower termite symbiotic system and ligno-cellulose digestion
English
Bauwens, Julien mailto [Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive >]
Brasseur, Catherine mailto [Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
Tarayre, Cédric mailto [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
Millet, Catherine []
Destain, Jacqueline mailto [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
Vandenbol, Micheline mailto [Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique >]
Thonart, Philippe mailto [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
Portetelle, Daniel mailto [Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique >]
De Pauw, Edwin mailto [Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) >]
Haubruge, Eric mailto [Université de Liège - ULiège > > Premier Vice-Recteur >]
Francis, Frédéric mailto [Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive >]
Aug-2014
A0
Yes
No
International
10th European Congress of Entomology
du 03 au 08 août 2014
[en] Termite gut ; Fractionation ; Lignocellulose ; Bacteria ; Flagellates ; Metaproteomics ; Multi-Lectin Affinity Chromatography
[en] Wood-feeding termites are a considerable source of enzymes active on ligno-cellulosic compounds. These enzymes are produced by the termite host and some representatives of its symbiotic microbial community, and are of particular interest in regard second generation biofuel. However, the complexity of microbial interactions renders micro-organisms isolation very difficult. Culture-independent methods permitted to gather a large amount of data and to understand a little more the role of each microbial population, particularly the prokaryotes. Proteomics allows working on the final product of gene expression, and corresponds more to the real operation of the digestive system. In order to investigate such a complex system, it is necessary to use multidisciplinary approaches and to fractionate this system. Zymography or affinity chromatography are used in parallel of routine proteomics techniques such as two-dimensional gel electrophoresis associated to MALDI-TOF mass spectrometry and nano-LC ESI-MS/MS. We used an artificial-diet based rearing to induce changes in microbial population balance. We performed preliminary assay to investigate the glycosylated proteome in the hindgut of a lower termite, using Multi-Lectin Affinity Chromatography (M-LAC) and enzymatic activity of harvested fractions was assessed on cellulosic substrates.
http://hdl.handle.net/2268/173197

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