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Development of entomopathogenic fungi in mosquito control: which kind of production for which efficiency?
Bawin, Thomas; Delvigne, Frank; Francis, Frédéric
2014International Congress on Invertebrate Pathology and Microbial Control
 

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Keywords :
Mosquito control; Entomopathogenic fungi; Bioreactor design; Solid-state fermentation; Biofilm fermentation
Abstract :
[en] Mosquitoes (Diptera: Culicidae) are zoonotic vectors responsible for numerous infectious diseases of medical and veterinary importance such as filariasis, malaria and encephalitis. As part of an integrated vector control, entomopathogenic fungi could be developed as biopesticides in two ways: spores and metabolites recognized as effective virulence factors. Solid-state fermentation enhances spore production and induces the secretion of metabolites quantitatively and qualitatively different from submerged fermentation, which impairs fungal metabolic efficiency. In this context, we showed high spore productivity of solid-state media based on agro-industrial substrates as wheat bran. Spores remained pathogenic, as revealed by classical toxicity tests and electron microscopy. However, the absence of free water makes culture parameter variations difficult to control in large-scale. Recently, we performed a bioreactor design intended for simultaneous spore and metabolite production, combining the technological advantages of submerged and solid-state fermentations. Biofilm fermentation (i.e. growth of fungal biomass on an inert support immerged in a nutrient medium) is a tremendous production system favouring the secretion of insecticidal metabolites in the liquid medium as we showed recently. This is also an interesting tool to provide an overview of the complexity of the metabolic pathways involved in the regulation of extracellular metabolites secretion because corresponding genes are reported to be differentially expressed from classical fermentation systems. Researches in vector control are currently intensified. In this context, the identification of genes and metabolites specifically expressed during biofilm fermentation will help to develop new technologies related both to the design of bioreactor and the production of insecticidal proteins.
Disciplines :
Biotechnology
Microbiology
Entomology & pest control
Author, co-author :
Bawin, Thomas ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Delvigne, Frank  ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Francis, Frédéric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Development of entomopathogenic fungi in mosquito control: which kind of production for which efficiency?
Publication date :
06 August 2014
Event name :
International Congress on Invertebrate Pathology and Microbial Control
Event organizer :
Society for Invertebrate Pathology
Event place :
Mainz, Germany
Event date :
3rd to 7th August 2014
Audience :
International
Available on ORBi :
since 20 August 2014

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