Reactivity towards singlet oxygen of propofol inside liposomes and neuronal cells

[en] Singlet oxygen (1O2), a reactive oxygen species, has been found to be implicated in many cellular events and pathological disorders.Herein, we investigated the reactivity of 1O2 towards the anaesthetic agent propofol (PPF) encapsulated within DMPC liposomes. By time resolved luminescence, the rate constant of 1O2 quenching by PPF was evaluated, depending on the location of the sensitizer. The nature of the oxidation product, resulting from the reaction of 1O2 with PPF, was determined using absorption and HPLC techniques. Finally, the in vitro protective effect of PPF towards the1O2-induced neuronal cell toxicity was evaluated in terms of cell viability.

Disciplines :

Physics

Author, co-author :

Heyne, Belinda

Brault, Daniel

Fontaine-Aupart, Marie-Pierre

Kohnen, Stéphane

Tfidel, Francis

Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Dupont, Ginette

Hoebeke, Maryse ; Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale

Language :

English

Title :

Reactivity towards singlet oxygen of propofol inside liposomes and neuronal cells

Publication date :

June 2005

Journal title :

Biochimica et Biophysica Acta - General Subjects

ISSN :

0304-4165

eISSN :

1872-8006

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

1724

Pages :

100-107

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 30 April 2010

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