Mitoproteome plasticity of rat brown adipocytes in response to cold acclimation

[en] Cold acclimation induces an adaptative increase in respiration in brown adipose tissue (BAT). A comparative analysis by two-dimensional differential in-gel electrophoresis of mitochondrial protein patterns found in rat control and cold-acclimated BAT was performed. A total of 58 proteins exhibiting significant differences in their abundance was unambiguously identified. Proteins implicated in the major catabolic pathways were up-regulated as were ATP synthase and mitofilin. Moreover, these results support the fact that adipocytes can balance their ATP synthesis and their heat production linked to UCP1-sustained uncoupling.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Navet, Rachel ; Université de Liège - ULiège > GIGA - Formation

Mathy, Grégory ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire

Douette, Pierre ; Université de Liège - ULiège > Bioénergétique et physiologie cellulaire

Dobson, Rowan ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie physique, spectrométrie de masse

Leprince, Pierre ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.

De Pauw, Edwin ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse

Sluse-Goffart, Claudine; Université de Liège - ULiège

Sluse, Francis ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire

Language :

English

Title :

Mitoproteome plasticity of rat brown adipocytes in response to cold acclimation

Publication date :

05 January 2007

Journal title :

Journal of Proteome Research

ISSN :

1535-3893

eISSN :

1535-3907

Publisher :

Amer Chemical Soc, Washington, United States - Washington

Volume :

Issue :

Pages :

25-33

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 01 December 2008

Statistics

Number of views

100 (12 by ULiège)

Number of downloads

7 (7 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Nicholls, D. G.; Locke, R. M. Thermogenic mechanisms in brown fat. Physiol. Rev. 1984, 64, 1-64.
Cannon, B.; Nedergaard, J. Brown adipose tissue: function and physiological significance. Physiol. Rev. 2003, 84, 277-359.
Nicholls, D. G.; Rial, E. A history of the first uncoupling protein, UCP1. J. Bioenerg. Biomembr. 1999, 31, 399-406.
Klingenberg, M.; Echtay, K. S. Uncoupling proteins: the issues from a biochemist point of view. Biochim. Biophys. Acta. 2001, 1504, 128-143.
Vercesi, A. E.; Martins, I. S.; Silva, M. A. P.; Leite, H. M. L. PUMPing plants. Nature 1995, 375, 24.
Ricquier, D.; Bouillaud, F. The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP. Biochem. J. 2000, 345, 161-179.
Jarmuszkiewicz, W.; Sluse-Goffart, C. M.; Hryniewiecka, L.; Sluse, F. E. Identification and characterization of a protozoan uncoupling protein in Acanthamoeba castellanii. J. Biol. Chem. 1999, 274, 23198-23202.
Jarmuszkiewicz, W.; Milani, G.; Fortes, F.; Schreiber, A. Z.; Sluse, F. E.; Vercesi, A. E. First evidence and characterization of an uncoupling protein in fungi kingdom: CpUCP of Candida parapsilosis. FEBS Lett. 2000, 467, 145-149.
Jarmuszkiewicz, W.; Behrendt, M.; Navet, R.; Sluse, F. E. Uncoupling protein and alternative oxidase of Dictyostelium discoideum: occurrence, properties and protein expression during vegetative life and starvation-induced early development. FEBS Lett. 2002, 532, 459-464.
Ledesma, A.; de Lacoba, M. G.; Rial, E. The mitoctiondrial uncoupling proteins. GenomeBiology 2002, 3 (12), 3015.
Argyropoulos, G.; Harper, M. E. Uncoupling proteins and thermoregulation. J. Appl. Physiol. 2002, 92, 2187-2198.
Sluse, F. E.; Jarmuszkiewicz, W. Uncoupling proteins outside the animal and plant kingdoms: functional and evolutionary aspects. FEBS Lett. 2002, 510, 117-120.
Douette, P.; Sluse, E. F. Mitochondrial uncoupling proteins: new insights from functional and proteomic studies. Free Radical Biol. Med. 2006, 40, 1097-1107.
Lindberg, O.; de Pierre, J.; Rylander, E.; Afzelius, B. A. Studies of the mitochondrial energy-transfer system of brown adipose tissue. J. Cell Biol. 1967, 34, 293-310.
Houstek, J.; Andersson, U.; Tvrdik, P.; Nedergaard, J.; Cannon, B. The expression of subunit c correlates with and thus may limit the biosynthesis of the mitochondrial F0F1-ATPase in brown adipose tissue. J. Biol. Chem. 1995, 270, 7689-7694.
Lin, C. S.; Klingenberg, M. Isolation of the uncoupling protein from brown adipose tissue mitochondria. FEBS Lett. 1980, 113, 299-303.
Douette, P.; Gerkens, P.; Navet, R.; Leprince, P.; De Pauw, E.; Sluse, F. E. Uncoupling protein 1 affects the yeast mitoproteome and oxygen free radical production. Free Radical Biol. Med. 2006, 40, 303-315.
Alban, A.; David, S. O.; Bjorkesten, L.; Andersson, C.; Sloge, E.; Lewis, S.; Currie, I. A novel experimental design for comparative two-dimensional gel analysis: two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics 2003, 3, 36-44.
Knowles, M. R.; Cervino, S.; Skynner, H. A.; Hunt, S. P.; de Felipe, C.; Salim, K.; Meneses-Lorente, G.; McAllister, G.; Guest, P. C. Multiplex proteomic analysis by two-dimensional differential ingel electrophoresis. Proteomics 2003, 3, 1162-1171.
Gorg, A.; Boguth, G.; Obermaier, C.; Posch, A.; Weiss, W. Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal systems. Electrophoresis 1995, 16, 1079-1086.
Karas, M.; Hillenkamp, F. Laser desorption ionization of proteins with molecular mass exceeding 1000 Dalton. Anal. Chem. 1988, 60, 2299-2301.
Mathy, G.; Navet, R.; Gerkens, P.; Leprince, P.; De Pauw, E.; Sluse-Goffart, C.; Sluse, F.; Douette, P. Saccharomyces cerevisiae mitoproteome plasticity in response to recombinant alternative ubiquinol oxidase. J. Proteome Res. 2006, 5, 339-348.
Douette, P.; Navet, R.; Gerkens, P.; de Pauw, E.; Leprince, P.; Sluse-Goffart, C.; Sluse, F. E. Steatosis-induced proteomic changes in liver mitochondria evidenced by two-dimensional differential in-gel electrophoresis. J. Proteome Res. 2005, 4, 2024-2031.
Zaninovich, A.; Raices, M.; Rebagliati, I.; Ricci, C.; Hagmüller, K. Brown fat thermogenesis in cold-acclimated rats is not abolished by the suppression of thyroid function. Am. J. Physiol. Endocrinol. Metab. 2002, 283, E496-E502.
Eaton, S. Control of mitochondrial beta-oxidation flux. Prog. Lipid Res. 2002, 41, 197-239.
Scholte, H. R. The intracellular and intramitochondrial distribution of malonyl-CoA decarboxylase and propionyl-CoA carboxylase in rat liver. Biochim. Biophys. Acta. 1969, 178, 137-144.
Frerman, F. E. Reaction of electron-transfer flavoprotein ubiquinone oxidoreductase with the mitochondrial respiratory chain. Biochim. Biophys. Acta. 1987, 893, 161-169.
Kedishvili, N. Y.; Popov, K. M.; Jaskiewicz, J. A.; Harris, R. A. Coordinated expression of valine catabolic enzymes during adipogenesis: analysis of activity, mRNA, protein levels, and metabolic consequences. Arch. Biochem. Biophys. 1994, 315, 317-322.
Grinblat, L.; Pacheco, Bolanos, L. F.; Stoppani, A. O. Decreased rate of ketone-body oxidation and decreased activity of D-3-hydroxybutyrate dehydrogenase and succinyl-CoA:3-oxo-acid CoA-transferase in heart mitochondria of diabetic rats. Biochem. J. 1986, 240, 49-56.
Sazanov, L. A.; Jackson, J. B. Proton-translocating transhydrogenase and NAD- and NADP-linked isocitrate dehydrogenases operate in a substrate cycle which contributes to fine regulation of the tricarboxylic acid cycle activity in mitochondria. FEBS Lett. 1994, 344, 109-116.
Gasparetti, A. L.; de Souza, C. T.; Pereira-da-Silva, M.; Oliveira, R. L.; Saad, M. J.; Carneiro, E. M.; Velloso, L. A. Cold exposure induces tissue-specific modulation of the insulin-signalling pathway in Rattus norvegicus. J. Physiol. 2003, 552, 149-162.
John, G. B.; Shang, Y.; Li, L.; Renken, C.; Mannella, C. A.; Selker, J. M.; Rangell, L.; Bennett, M. J.; Zha, J. The mitochondrial inner membrane protein mitofilin controls cristae morphology. Mol. Biol. Cell 2005, 16, 1543-1554.
Mannella, C.; Pfeiffer, D.; Bradshaw, P.; Moraru, I.; Slepchenko, B.; Loew, L.; Hsich, C.; Buttle, K.; Marko, M. Topology of the mitochondrial inner membrane: dynamics and bioenergetic implication. IUBMB Life 2001, 52, 93-100.
Daugas, E.; Nochy, D.; Ravagnan, L.; Loeffler, M.; Susin, S. A.; Zamzami, N.; Kroemer, G. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett. 2000, 476, 118-123.
Wilson-Fritch, L.; Nicoloro, S.; Chouinard, M.; Lazar, M. A.; Chui, P. C.; Leszyk, J.; Straubhaar, J.; Czech, M. P.; Corvera, S. Mitochondrial remodelling in adipocite tissue associated with obesity and treatment with rosiglitazone. J. Clin. Invest. 2004, 114, 1281-1289.