Reference : Spatial optimization in perfusion bioreactors improves bone tissue-engineered constru...
Scientific journals : Article
Engineering, computing & technology : Multidisciplinary, general & others
http://hdl.handle.net/2268/168789
Spatial optimization in perfusion bioreactors improves bone tissue-engineered construct quality attributes
English
Papantoniou, Ioannis [KULeuven > Prometheus, Division of Skeletal Tissue Engineering > Skeletal Biology and Engineering Research Center > >]
Guyot, Yann mailto [Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique >]
Sonnaert, Maarten [KULeuven > Prometheus, Division of Skeletal Tissue Engineering > Department of Metallurgy and Materials Engineering > >]
Kerckhofs, Greet mailto [Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique >]
Luyten, Franck P. [KULeuven > Prometheus, Division of Skeletal Tissue Engineering > Skeletal Biology and Engineering Research Center > >]
Geris, Liesbet mailto [Université de Liège - ULiège > Département d'aérospatiale et mécanique > Génie biomécanique >]
Schrooten, Jan [KULeuven > Prometheus, Division of Skeletal Tissue Engineering > Department of Metallurgy and Materials Engineering > >]
Jun-2014
Biotechnology and Bioengineering
John Wiley & Sons, Inc
Yes (verified by ORBi)
International
0006-3592
1097-0290
[en] perfusion bioreactor ; issue engineered construct ; contrast enhanced nanoCT ; quality attributes ; human periosteum derived stem cells
[en] Perfusion bioreactors have shown great promise for tissue engineering applications providing a homogeneous and consistent distribution of nutrients and flow-induced shear stresses throughout tissue-engineered constructs. However, non uniform fluid-flow profiles found in the perfusion chamber entrance region have been shown to affect tissue-engineered construct quality characteristics during culture. In this study a whole perfusion and construct, three dimensional (3D) computational fluid dynamics approach was used in order to optimize a critical design parameter such as the location of the regular pore scaffolds within the perfusion bioreactor chamber. Computational studies were coupled to bioreactor experiments for a case-study flow rate. Two cases were compared in the first instance seeded scaffolds were positioned immediately after the perfusion chamber inlet while a second group was positioned at the computationally determined optimum distance were a steady state flow profile had been reached. Experimental data showed that scaffold location affected significantly cell content and neo-tissue distribution, as determined and quantified by contrast enhanced nanoCT, within the constructs both at 14 and 21 days of culture. However gene expression level of osteopontin and osteocalcin was not affected by the scaffold location. This study demonstrates that the bioreactor chamber environment, incorporating a scaffold and its location within it, affects the flow patterns within the pores throughout the scaffold requiring therefore dedicated optimization that can lead to bone tissue engineered constructs with improved quality attributes
http://hdl.handle.net/2268/168789
10.1002/bit.25303

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