[en] A generic method was developed for the liquid chromatographic (LC) assay of six corticosteroids and salicylic acid in different dermatological formulations containing methylparaben and propylparaben or sorbic acid as preservatives. An adequate separation was obtained using octadecylsilica as stationary phase and a mixture of acetonitrile (MeCN) and 25 mM phosphate buffer of pH 3.5 as mobile phase. Segmented gradient elution was applied from 30 to 45% of MeCN in 9.9 minutes, then from 45 to 96% in 19.0 minutes. UV detection was performed at 240 nm. The method was validated. Very good results were obtained with respect to linearity selectivity, precision and accuracy.
Reynolds, J.E.F., Parfitt, K., Parsons, A.V., Sweetman, S.C., (1996) Corticosteroids in Martindale, The Extra Pharmacopoeia, 31st Edition, , The Royal Pharmaceutical Society, London
Mutschler, E., Derendorf, H., (1995) Drug Actions: Basic Principles and Therapeutic Aspects, , Medpharm, Stuttgart
Nichols, W.K., (1995) The Science and Practice of Pharmacy, 2. , Gennaro, A.R., Ed, Remington, 19th Edition, Mack Publishing Company, Easton
Schorderet, M., (1998) Des Concepts fondamentaux aux applications thérapeutiques, Pharmacologie 3ème Edition, , Frison-Roche, Paris
Angelillo, B., Boccia, A., (1997) Pharmatherapeutica, 1, p. 434
Blanco, M., Coello, J., Iturriaga, H., Maspoch, S., Illegas, N., (1999) Analyst, 124, pp. 911-915
Bonazzi, D., Andrissano, V., Gatti, R., Cavrini, V., (1995) J. Pharm. Biomed. Anal., 13, pp. 1321-1329
Bonazzi, D., Gotti, R., Gatti, R., Cavrini, V., (1997) Farmaco, 52, pp. 193-197
