Article (Scientific journals)
Varicella-zoster virus induces the formation of dynamic nuclear capsid aggregates.
Lebrun, Marielle; Thelen, Nicolas; Thiry, Marc et al.
2014In Virology, 454-455, p. 311-27
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Keywords :
Assembly; Capsid; Correlative microscopy; Fluorescent virus; Herpesvirus; ORF23; UL36; UL37; VP26; VZV
Abstract :
[en] The first step of herpesviruses virion assembly occurs in the nucleus. However, the exact site where nucleocapsids are assembled, where the genome and the inner tegument are acquired, remains controversial. We created a recombinant VZV expressing ORF23 (homologous to HSV-1 VP26) fused to the eGFP and dually fluorescent viruses with a tegument protein additionally fused to a red tag (ORF9, ORF21 and ORF22 corresponding to HSV-1 UL49, UL37 and UL36). We identified nuclear dense structures containing the major capsid protein, the scaffold protein and maturing protease, as well as ORF21 and ORF22. Correlative microscopy demonstrated that the structures correspond to capsid aggregates and time-lapse video imaging showed that they appear prior to the accumulation of cytoplasmic capsids, presumably undergoing the secondary egress, and are highly dynamic. Our observations suggest that these structures might represent a nuclear area important for capsid assembly and/or maturation before the budding at the inner nuclear membrane.
Research center :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Microbiology
Author, co-author :
Lebrun, Marielle  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie et immunologie
Thelen, Nicolas  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Riva, Laura ;  Université de Liège - ULiège > R&D Direction : Chercheurs ULiège en mobilité
Ote, Isabelle 
Condé, Claude 
Vandevenne, Patricia 
Di Valentin, Emmanuel  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie et immunologie
BONTEMS, Sébastien 
Sadzot-Delvaux, Catherine ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie et immunologie
Language :
English
Title :
Varicella-zoster virus induces the formation of dynamic nuclear capsid aggregates.
Publication date :
2014
Journal title :
Virology
ISSN :
0042-6822
eISSN :
1096-0341
Publisher :
Elsevier, Atlanta, United States - California
Volume :
454-455
Pages :
311-27
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2014 Elsevier Inc. All rights reserved.
Available on ORBi :
since 23 May 2014

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