Influence of medium composition on oxygen transfer rate in animal cell culture

Toye, Dominique; Galifi, A.; Salmon, Thierry; Marchot, Pierre; Verdin, Emeline; Crine, Michel

doi:10.1002/cjce.20302

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Article (Scientific journals)

Influence of medium composition on oxygen transfer rate in animal cell culture

Toye, Dominique; Galifi, A.; Salmon, Thierry et al.

2010 • In Canadian Journal of Chemical Engineering, 88 (4), p. 671-676

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https://hdl.handle.net/2268/16722

DOI
10.1002/cjce.20302

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Keywords :

kLa; interfacial area; bubble diameter; optical probe; disengagement technique

Abstract :

[en] Experiments were conducted in a 0.25m diameter bubble column to investigate the effect of medium composition on oxygen transfer rate. Aqueous solutions, the composition of which mimics a mammalian cell culture medium, are used. The effect on oxygen transfer rate of additives used to protect cells against local hydrodynamic stresses induced by bubble coalescence and bursting is addressed, in the range of operating conditions (aeration rates) met in animal cell cultures. The mass transfer coefficient, the liquid viscosity and surface tension, and the bubble size distribution are measured as a function of liquid composition and of gas superficial velocity, allowing to decouple the effects of the different additive on kL and on a.

Disciplines :

Chemical engineering

Author, co-author :

Toye, Dominique ; Université de Liège - ULiège > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques

Galifi, A.

Salmon, Thierry ; Université de Liège - ULiège > Département de chimie appliquée > Département de chimie appliquée

Marchot, Pierre ; Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Systèmes polyphasiques

Verdin, Emeline ; Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Opérations physiques unitaires

Crine, Michel ; Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Opérations physiques unitaires

Language :

English

Title :

Influence of medium composition on oxygen transfer rate in animal cell culture

Publication date :

2010

Journal title :

Canadian Journal of Chemical Engineering

ISSN :

0008-4034

Publisher :

Chemical Institute of Canada, Ottawa, Canada

Volume :

Issue :

Pages :

671-676

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 January 2010

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