Reference : Multilocus sequence typing analysis and antibiotic resistance of Clostridium difficil...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/165988
Multilocus sequence typing analysis and antibiotic resistance of Clostridium difficile strains isolated from retail meat and humans in Belgium
English
Rodriguez Diaz, Cristina mailto [Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Technologie des denrées alimentaires >]
Taminiau, Bernard mailto [Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires >]
Avesani, Véronique [Catholic University of Louvain, Brussels > Microbiology Unit > > >]
Van Broeck, Johan [Catholic University of Louvain, Brussels > Microbiology Unit > > >]
Delmée, Michel [Catholic University of Louvain, Brussels > Microbiology Unit > > >]
Daube, Georges mailto [Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires >]
Sep-2014
Food Microbiology
Elsevier
42
166-171
Yes (verified by ORBi)
International
0740-0020
1095-9998
London
United Kingdom
[en] Clostridium difficile ; retail meat ; multilocus sequence typing analysis
[en] Clostridium difficile has been isolated from food animals and meat, specially ground pork and ground beef. The recovered isolates were closely related to C. difficile human strains, indicating that animals and food are possible transmission routes of human C. difficile infection. The main objective of this study was to characterize C. difficile isolates from retail meat and to compare with human isolates recovered from hospital patients in Belgium. Raw meat (beef and pork) was obtained from the retail trade. C. difficile was recovered from 2.3% of the beef samples and from 4.7% of the pork samples. A total of 4 different PCR-ribotypes were identified with a large percentage of types 078 and 014. Resistance to moxifloxacin and erythromycin was detected. The multi-locus sequence typing (MLST) analysis showed that meat and human isolates cluster in the same lineage. This study reveals the presence of toxigenic C. difficile in retail meat in Belgium with predominance PCR-ribotypes 078 and 014, which are among the four most prevalent ribotypes of C. difficile isolated from humans in Europe.
Federal Public Service of Health, Food Chain Safety and Environment (contract RF09/6226).
http://hdl.handle.net/2268/165988

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
1-s2.0-S0740002014000744-main.pdfPublisher postprint451.3 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.