Reference : Respiratory-deficient mutants of the unicellular green alga Chlamydomonas: A review.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/163710
Respiratory-deficient mutants of the unicellular green alga Chlamydomonas: A review.
English
Salinas, Thalia [> >]
Larosa, Véronique mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues >]
Cardol, Pierre mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues >]
Marechal-Drouard, Laurence [> >]
Remacle, Claire mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues >]
2013
Biochimie
Yes (verified by ORBi)
International
0300-9084
1638-6183
[en] Chlamydomonas ; Mitochondria ; Respiratory chain ; Respiratory-deficient mutants
[en] Genetic manipulation of the unicellular green alga Chlamydomonas reinhardtii is straightforward. Nuclear genes can be interrupted by insertional mutagenesis or targeted by RNA interference whereas random or site-directed mutagenesis allows the introduction of mutations in the mitochondrial genome. This, combined with a screen that easily allows discriminating respiratory-deficient mutants, makes Chlamydomonas a model system of choice to study mitochondria biology in photosynthetic organisms. Since the first description of Chlamydomonas respiratory-deficient mutants in 1977 by random mutagenesis, many other mutants affected in mitochondrial components have been characterized. These respiratory-deficient mutants increased our knowledge on function and assembly of the respiratory enzyme complexes. More recently some of these mutants allowed the study of mitochondrial gene expression processes poorly understood in Chlamydomonas. In this review, we update the data concerning the respiratory components with a special focus on the assembly factors identified on other organisms. In addition, we make an inventory of different mitochondrial respiratory mutants that are inactivated either on mitochondrial or nuclear genes.
http://hdl.handle.net/2268/163710
10.1016/j.biochi.2013.10.006
Copyright (c) 2013 Elsevier Masson SAS. All rights reserved.

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