Poster (Scientific congresses and symposiums)
Biofilms from entomopathogenic fungi in mosquito control
Bawin, Thomas; Boukraa, Slimane; Seye, Fawrou et al.
201419th National Symposium on Applied Biological Sciences
 

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Keywords :
Mosquito control; Entomopathogenic fungi; Biofilm; Metabolites
Abstract :
[en] Mosquitoes (Diptera: Culicidae) are zoonotic vectors of medical and veterinary importance. As part of an integrated vector control, metabolites secreted by entomopathogenic fungi could be developed as biopesticides. In this context, filamentous microorganisms growing on a support as biofilm in a liquid medium would offer several advantages in bioreactor regarding performances and metabolites recovery. The production of toxic metabolites by an entomopathogenic fungus Aspergillus flavus in such conditions was assessed. Three initial inoculum levels, i.e. 10^1, 10^3 and 10^6 spores/ml of PYG medium, have been tested in shake flask with or without support. Toxicity tests were performed on Culex quinquefasciatus larvae using dilutions of 1, 2, 4, 6, 8 and 10% of liquid cultures. The results indicated that A. flavus tends to form pellets in submerged culture; the size and the amount of pellets was affected by the initial inoculum level of spores. Under similar conditions, the filaments fixed on a support and didn’t appear in free form in the liquid. Toxicity tests revealed differences between both free and fixed forms. All combined conditions, LC50s ranging up to dilutions of 2.2 and 4.8% were observed within 48 hours. Secretomes could be compared between these culture conditions by proteomic and metabolomic approaches.
Disciplines :
Entomology & pest control
Biotechnology
Author, co-author :
Bawin, Thomas ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive > Doct. sc. agro. & ingé. biol.
Boukraa, Slimane;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive > Doct. sc. agro. & ingé. biol.
Seye, Fawrou;  Université Cheik Anta Diop > Sciences et technologies > Laboratoire de biologie de la reproduction
Raharimalala, Fara Nantenaina ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Zimmer, Jean-Yves ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive > Doct. sc. agro. & ingé. biol.
Delvigne, Frank  ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Francis, Frédéric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Biofilms from entomopathogenic fungi in mosquito control
Publication date :
07 February 2014
Event name :
19th National Symposium on Applied Biological Sciences
Event place :
Gembloux, Belgium
Event date :
7 février 2014
Available on ORBi :
since 08 February 2014

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