Fermentation; Forage; Nutritive value; Pigs; Short chain fatty acids
Abstract :
[en] The nutritive value of 20 forage plants commonly used for feeding pigs in the Democratic
Republic of the Congo was studied to determine chemical composition, protein amino acid
profiles, mineral content, and in vitro digestibility using a two-step method combining an
enzymatic pepsin and pancreatin hydrolysis followed by a 72 h gas-test fermentation. The
highest protein contents (270–320 g/kg DM) were obtained for Vigna unguiculata, Psophocarpus
scandens, Leucaena leucocephala, Manihot esculenta, and Moringa oleifera. Grasses,
Acacia mangium, and Eichhornia crassipes, showed the lowest crude protein (CP) and highest
NDF contents. Cajanus cajan and Trypsacum andersonii had the most balanced amino
acid profile, being deficient in lysine and slightly deficient in histidine, while Megathyrsus
maximus displayed the highest number of essential amino acids deficiencies. High mineral
contents were obtained from, in ascending order, with M. oleifera, V. unguiculata, E. crassipes,
Ipomea batatas and Amaranthus hybridus. In vitro dry matter digestibility ranged from
0.25 to 0.52, in vitro CP digestibility from 0.23 to 0.80, in vitro energy digestibility from 0.23
to 0.52. M. esculenta, M. oleifera, I. batatas, Mucuna pruriens, V. unguiculata, P. scandens and A. hybridus showed high digestibilities for all nutrients. Gas production during fermentation
of the pepsin and pancreatin-indigestible fraction of the plants varied from 42 ml/g DM
for A. mangium to 202 ml/g DM for I. batatas (P<0.001). Short-chain fatty acid production
during fermentation varied from 157 to 405 mg/g of the pepsin and pancreatin indigestible
fraction. It is concluded that some of these species are interesting sources of proteins and
minerals with a good digestibility that might be used more economically than concentrate,
especially in smallholder production systems, to improve pig feeding, mineral intake and
intestinal health in pigs reared in the tropics.
Acamovic, T., Brooker, J.D., Biochemistry of plant secondary metabolites and their effects in animals (2005) Proc. Nutr. Soc., 64, pp. 403-412
Agte, V.V., Tarwadi, K.V., Mengale, S., Chiplonkar, S.A., Potential of traditionally cooked green leafy vegetables as natural sources for supplementation of eight micronutrients in vegetarian diets (2000) J. Food Comp. Anal., 13, pp. 885-891
An, L.V., Hong, T.T.T., Lindberg, J.E., Ileal and total tract digestibility in growing pigs fed cassava root meal diets with inclusion of fresh, dry and ensiled sweet potato (Ipomoea batatas L. (Lam.)) leaves (2004) Anim. Feed Sci. Technol., 114, pp. 127-139
(1990) Official Methods of Analysis, , Association Official Analytical Chemists, Arlington, A.O.A. Chemists (Ed.)
Bindelle, J., Buldgen, A., Boudry, C., Leterme, P., Effect of inoculum and pepsin-pancreatin hydrolysis on fibre fermentation measured by the gas production technique in pigs (2007) Anim. Feed Sci. Technol., 132, pp. 111-122
Bindelle, J., Buldgen, A., Wavreille, J., Agneessens, R., Destain, J.P., Wathelet, B., Leterme, P., The source of fermentable carbohydrates influences the in vitro protein synthesis by colonic bacteria isolated from pigs (2007) Animal, 1, pp. 1126-1133
Braun, J.V., (2007) The World Food Situation: New Driving Forces and Required Actions, , International Food Policy Research Institute, Washington, D.C
Cherrington, C.A., Hinton, M., Pearson, G.R., Chopra, I., Short-chain organic acids at pH 5.0 kill Escherichia coli and Salmonella spp. without causing membrane perturbation (1991) J. Appl. Microbiol., 70, pp. 161-165
Clavero, T., Razz, R., Chemical compositions and anti-nutritive factors of Acacia mangium (2011) J. Anim. Sci., , 89, E-Suppl. 1/J., 46
(2012) Food Price Index, , World Food Situation, FAO
Gaedeken, D., Breves, G., Oslage, H.J., Efficiency of energy utilization of intracaecally infused volatile fatty acids in pigs (1989) Symposium on energy metabolism of farm animals, 11, pp. 115-118. , Pudoc, Wageningen (Netherlands)
Groot, J.C.J., Cone, J.W., Williams, B.A., Debersaques, F.M.A., Lantinga, E.A., Multiphasic analysis of gas production kinetics for in vitro fermentation of ruminant feeds (1996) Anim. Feed Sci. Technol., 64, pp. 77-89
Hijova, E., Chmelarova, A., Short chain fatty acids and colonic health (2007) Bratisl. Lek. Listy, 108, pp. 354-358
Kaensombath, L., Lindberg, J.E., Effect of replacing soybean protein by taro leaf (Colocasia esculenta (L.) Schott) protein on growth performance of exotic (Landrace x Yorkshire) and native (Moo Lath) Lao pigs (2013) Trop. Anim. Health Prod., 45, pp. 45-51
Kaensombath, L., Neil, M., Lindberg, J.E., Effect of replacing soybean protein with protein from ensiled stylo (Stylosanthes guianensis (Aubl.) Sw. var. guianensis) on growth performance, carcass traits and organ weights of exotic (Landrace x Yorkshire) and native (Moo Lath) Lao pigs (2013) Trop. Anim. Health Prod., 45, pp. 865-871
Kumaresan, A., Bujarbaruah, K.M., Pathak, K.A., Das, A., Bardoloi, R.K., Integrated resource-driven pig production systems in a mountainous area of Northeast India: production practices and pig performance (2009) Trop. Anim. Health Prod., 41, pp. 1187-1196
Kumari, M., Gupta, S., Lakshmi, A.J., Prakash, J., Iron bioavailability in green leafy vegetables cooked in different utensils (2004) Food Chem., 86, pp. 217-222
Le Goff, G., Milgen, J., Noblet, J., Influence of dietary fibre on digestive utilization and rate of passage in growing pigs, finishing pigs and adult sows (2002) Anim. Sci., 74, pp. 503-515
Lemke, U., Valle Zárate, A., Dynamics and developmental trends of smallholder pig production systems in North Vietnam (2008) Agric. Syst., 96, pp. 207-223
Leterme, P., Londoño, A.M., Estrada, F., Souffrant, W.B., Buldgen, A., Chemical composition, nutritive value and voluntary intake of tropical tree foliage and cocoyam in pigs (2005) J. Sci. Food Agric., 85, pp. 1725-1732
Leterme, P., Boteroa, M., Londoñoa, A.M., Bindelle, J., Buldgen, A., Nutritive value of tropical tree leaf meals in adult sows (2006) Anim. Sci., 82, pp. 175-182
Liu, J., Bollinger, D.W., Ledoux, D.R., Venum, T.L., Effects of dietary calcium: phosphorus ratios on apparent absorption of calcium and phosphorus in the small intestine, cecum, and colon of pigs (2000) J. Anim. Sci., 78, pp. 106-109
Livesey, G., Elia, M., Short-chain fatty acids as an energy source in the colon: metabolism and clinical implications (1995) Physiological and clinical aspects of short-chain fatty acids, pp. 427-481. , Cambridge University Press, UK, J.H. Cummings, J.L. Rombeau, T. Sakata (Eds.)
Martens, S.D., Tiemann, T.T., Bindelle, J., Peters, M., Lascano, C.E., Alternative plant protein sources for pigs and chickens in the tropics-nutritional value and constraints: a review (2012) J. Agric. Rural Dev. Trop., 113, pp. 101-123
Men, L.T., Yamasaki, S., Caldwell, J.S., Yamada, R., Takada, R., Taniguchi, T., Effect of farm household income levels and rice-based diet or water hyacinth (Eichhornia crassipes) supplementation on growth/cost performances and meat indexes of growing and finishing pigs in the Mekong Delta of Vietnam (2006) Anim. Sci. J., 77, pp. 320-329
Noblet, J., van Milgen, J., Energy value of pig feeds: effect of pig body weight and energy evaluation system (2004) J. Anim. Sci., 82, pp. 229-238
(2012) Nutrient Requirements of Swine, , National Academy Press, Washington, DC
Partanen, K., Jalava, T., Valaja, J., Effects of a dietary organic acid mixture and of dietary fibre levels on ileal and faecal nutrient apparent digestibility, bacterial nitrogen flow, microbial metabolite concentrations and rate of passage in the digestive tract of pigs (2007) Animal, 1, pp. 389-401
Phengsavanh, P., Lindberg, J., Effect of replacing soybean protein with protein from porcupine joint vetch (Aeschynomene histrix BRA 9690) and stylo (Stylosanthes guianensis Composite) leaf meal on growth performance of native (Moo Lath) Lao pigs (2013) Trop. Anim. Health Prod., pp. 1-8
Phengsavanh, P., Ogle, B., Stur, W., Frankow-Lindberg, B.E., Lindberg, J.E., Feeding and performance of pigs in smallholder production systems in Northern Lao PDR (2010) Trop. Anim. Health Prod., 42, pp. 1627-1633
Phuc, B.H.N., Lindberg, J.E., Ileal and total tract digestibility in growing pigs given cassava root meal diets with inclusion of cassava leaves, leucaena leaves and groundnut foliage (2000) Anim. Sci., 71, pp. 301-308
Pieper, R., Bindelle, J., Malik, G., Marshall, J., Rossnagel, B.G., Leterme, P., Van Kessel, A.G., Influence of different carbohydrate composition in barley varieties on Salmonella Typhimurium var. Copenhagen colonisation in a Trojan challenge model in pigs (2012) Arch. Anim. Nutr., 66, pp. 163-179
Poulsen, H.D., Carlson, D., Nørgaard, J.V., Blaabjerg, K., Phosphorus digestibility is highly influenced by phytase but slightly by calcium in growing pigs (2010) Livest. Sci., 134, pp. 100-102
Régnier, C., Bocage, B., Archimède, H., Noblet, J., Renaudeau, D., Digestive utilization of tropical foliages of cassava, sweet potatoes, wild cocoyam and Erythrina in Creole growing pigs (2013) Anim. Feed Sci. Technol., 180, pp. 44-54
Régnier, C., Jaguelin, Y., Noblet, J., Renaudeau, D., Ileal digestibility of amino acids of cassava, sweet potato, cocoyam and erythrina foliages fed to growing pigs (2012) Animal, 6, pp. 586-593
Uwangbaoje, L.O., The mineral and phytochemical analysis of the leaves of senna alata and cajanus cajan and their medicinal value (2012) Int. J. Biol. Pharm. Allied Sci., 1, pp. 1-11
Van Soest, P.J., Robertson, J.B., Lewis, B.A., Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition (1991) J. Dairy Sci., 74, pp. 3583-3597