Intracellular pH-dependent efflux of the fluorescent probe pyranine in the yeast Yarrowia lipolytica

Octanoic acid; Probe leakage; Pyranine; Yarrowia lipolytica; Yeast intracellular pH; Arylsulfonates; Biological Transport; Cell Membrane Permeability; Cytoplasm; Fluorescent Dyes; Hydrogen-Ion Concentration; Octanoic Acids; Saccharomycetales

Abstract :

[en] 8-Hydroxypyrene-1,3,6-trisulfonic acid (pyranine) can be used as a vital intracellular pH (pHi) indicator. In the yeast Yarrowia lipolytica, a partial efflux of the probe was detected by using the pH-independent wavelength of 415 nm. A simplified correction of the fluorescent signals was applied, enabling to show for this species a good near-neutral pHi maintenance capacity in a pH 3.9 medium. Octanoic acid, which is known to have toxic effects on yeast, decreased the pHi and increased the 260-nm-absorbing compounds leakage. However, this acid inhibited the fluorescent probe efflux linearly with its concentration suggesting a pHi-dependent efflux of pyranine from cells. © 2001 Federation of European Microbiological Societies.

Disciplines :

Biotechnology

Author, co-author :

Aguedo, Mario ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle

Waché, Y.; Laboratoire De Biotechnologie, Equipe IMSA, Université De Bourgogne, 21000 Dijon, France

Belin, J.-M.; Laboratoire De Biotechnologie, Equipe IMSA, Université De Bourgogne, 21000 Dijon, France

Language :

English

Title :

Intracellular pH-dependent efflux of the fluorescent probe pyranine in the yeast Yarrowia lipolytica

Publication date :

2001

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Oxford University Press, United Kingdom

Volume :

200

Issue :

Pages :

185-189

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 05 February 2014

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