Reference : Isolation of an amylolytic chrysophyte, Poterioochromonas sp. from the digestive trac...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/162563
Isolation of an amylolytic chrysophyte, Poterioochromonas sp. from the digestive tract of the termite R. santonensis
English
[fr] Isolement d’une chrysophyte amylolytique, Poterioochromonas sp. de l’intestin du termite Reticulitermes santonensis
Tarayre, Cédric mailto [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
Bauwens, Julien mailto [Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive >]
Brasseur, Catherine mailto [Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Mattéotti, Christel []
Destain, Jacqueline mailto [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
Vandenbol, Micheline mailto [Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique >]
Portetelle, Daniel mailto [Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique >]
De Pauw, Edwin mailto [Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Haubruge, Eric mailto [Université de Liège - ULiège > > Vice-Recteur de Gembloux Agro Bio Tech >]
Francis, Frédéric mailto [Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive >]
Thonart, Philippe mailto [Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Jan-2014
Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE]
Presses Agronomiques de Gembloux
18
1
Yes (verified by ORBi)
International
1370-6233
1780-4507
Gembloux
Belgique
[en] Amylase ; Chrysophyte ; Culture ; Protiste ; Reticulitermes santonensis ; Termite
[en] The aim of this work was the isolation and cultivation of amylolytic protists living in the digestive tract of the termite Reticulitermes santonensis (Feytaud). A chrysophyte identified as Poterioochromonas sp. was isolated in a special medium containing rice grains as a source of carbon and nitrogen. Then, the protist was grown in a medium containing starch as a carbon source, tryptone, and a phosphate buffer at different pH values (5, 6 and 7). Yeast extract was added or not. Ciprofloxacin was used to avoid the bacterial development. Other antibiotics were also tested but showed an inhibitive effect on the growth of Poterioochromonas sp. Yeast extract allowed reaching 1.9 (pH 5), 2.3 (pH 6) and 2.2 (pH 7) times higher final cell concentrations, and 2.8 (pH 5), 2.8 (pH 6) and 2.2 (pH 7) times higher biomass yields. The starch concentration did not decrease in the medium until 3 and 4 days of culture, with and without yeast extract, respectively. Eight days of culture were necessary for hydrolyzing the starch completely, with and without yeast extract. Maltose and maltotriose were detected in the culture media and were hydrolyzed progressively. Maximal maltose concentrations were 0.68, 0.66 and 0.51 g.l-1 in the medium containing yeast extract. Maltotriose concentrations were only 0.17, 0.14 and 0.12 g.l-1. Other glucose oligomers were also detected but in lower quantities. It was determined that the protist developed a weak amylase activity, particularly at a weakly acidic pH (5-6). Such a pH also allowed a better growth of the protist. A maximal amylase activity of 112 nkat.l-1 was measured with yeast extract at pH 5. No other enzymatic activity (protease, cellulase or xylanase) was detected except amylase. The degradation products of starch which were obtained by enzymatic hydrolysis allow the identification of α-amylase, amyloglucosidase and possibly β-amylase activities.
Projet ARC Termitofuel
Researchers ; Professionals ; General public
http://hdl.handle.net/2268/162563

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