Proteomic characterization of murid herpesvirus 4 extracellular virions.

[en] Gammaherpesvirinae, such as the human Epstein-Barr virus (EBV) and the Kaposi's sarcoma associated herpesvirus (KSHV) are highly prevalent pathogens that have been associated with several neoplastic diseases. As EBV and KSHV are host-range specific and replicate poorly in vitro, animal counterparts such as Murid herpesvirus-4 (MuHV-4) have been widely used as models. In this study, we used MuHV-4 in order to improve the knowledge about proteins that compose gammaherpesviruses virions. To this end, MuHV-4 extracellular virions were isolated and structural proteins were identified using liquid chromatography tandem mass spectrometry-based proteomic approaches. These analyses allowed the identification of 31 structural proteins encoded by the MuHV-4 genome which were classified as capsid (8), envelope (9), tegument (13) and unclassified (1) structural proteins. In addition, we estimated the relative abundance of the identified proteins in MuHV-4 virions by using exponentially modified protein abundance index analyses. In parallel, several host proteins were found in purified MuHV-4 virions including Annexin A2. Although Annexin A2 has previously been detected in different virions from various families, its role in the virion remains controversial. Interestingly, despite its relatively high abundance in virions, Annexin A2 was not essential for the growth of MuHV-4 in vitro. Altogether, these results extend previous work aimed at determining the composition of gammaherpesvirus virions and provide novel insights for understanding MuHV-4 biology.

Disciplines :

Microbiology

Author, co-author :

Vidick, Sarah ; Université de Liège - ULiège > Immunologie et vaccinologie

Leroy, Baptiste

Palmeira, Leonor ; Université de Liège - ULiège > Département de productions animales > GIGA-R : Génomique animale

Machiels, Bénédicte ; Université de Liège - ULiège > Immunologie et vaccinologie

Mast, Jan

François, Sylvie ; Université de Liège - ULiège > Immunologie et vaccinologie

Wattiez, Ruddy

Vanderplasschen, Alain ; Université de Liège - ULiège > Immunologie et vaccinologie

Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie

Language :

English

Title :

Proteomic characterization of murid herpesvirus 4 extracellular virions.

Publication date :

2013

Journal title :

PLoS ONE

eISSN :

1932-6203

Publisher :

Public Library of Science, United States - California

Volume :

Issue :

Pages :

e83842

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 30 January 2014

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