Abstract :
[en] The production of essential oil by tissue culture s ol Antbemis nobilis L. including cell suspension, shoot and crown-gall cultures is reported. The biosynthetic capability of these different cultures was compared with that of plants grown in a field. The addition of crude polysaccharide fraction prepared from yeast extract and from the plant itself to
shoot cultures affected the composition and total essential content (from 0.08 o/o to 0.3Qo/o dry weight).
The essential oil content of the crown-gall tissue ol A. nobilis was 0.25 % of the dry weight and the composition of the essential oil was comparable to that of the flowers.
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