Dubensky TW, Campbell BA, Villarreal LP. Direct transfection of viral and plasmid DNA into the liver or spleen of mice. Proc Natl Acad Sci U S A 1984;81:7529-33; PMID:6095 303; http://dx.doi.org/10.1073/pnas.81.23.7529
Wolff JA, Malone RW, Williams P, Chong W, Acsadi G, Jani A, et al. Direct gene transfer into mouse muscle in vivo. Science 1990;247:1465-8; PMID: 1690918; http://dx. doi.org/10.112 6/science.1690918
Liu MA, Ulmer JB. Human clinical trials of plasmid DNA vaccines. Adv Genet 2005;55:25-40; PMID:16291211; http://dx.doi.org/10.1016/S0065-2660 (05) 55002-8
Heller R, Shirley S, Guo S, Donate A, Heller L. Electroporation Based Gene Therapy-from the Bench to the Bedside. Conference proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society IEEE Engineering in Medicine and Biology Society Conference 2011;2011:736-8.
Vandermeulen G, Athanasopoulos T, Trundley A, Foster K, Preat V, Yanez-Munoz RJ, et al. Highly potent delivery method of gpl60 envelope vaccine combining lentivirus-like particles and DNA electrotransfer. J Control Release 2012;159:376-83; PMID:22310089; http://dx.doi. Org/10.1016/j.jconrel.2012.01.035
Satkauskas S, Andre F, Bureau MF, Scherman D, Miklavcic D, Mir LM. Electrophoretic component of electric pulses determines the efficacy of in vivo DNA electro transfer. Hum Gene Ther 2005;16:1194-201; PMID:16218780; http://dx.doi.org/10.1089/hum.2005.16.1194
Liu MA, Wahren B, Karlsson Hedestam GB. DNA vaccines: recent developments and future possibilities. Hum Gene Ther 2006;17:1051-61; PMID:17032152; http://dx.doi.org/10.1089/hum.2006.17.1051
Moss RB. Prospects for control of emerging infectious diseases with plasmid DNA vaccines. J Immune Based Ther Vaccines 2009;7:3; PMID: 19735569; http://dx.doi.org/10.1186/1476-8518-7-3
Schalk JA, Mooi FR, Berbers GA, Van Aerts LA, Ovelgonne H, Kimman TG. Preclinical and clinical safety studies on DNA vaccines. Hum Vaccin 2006;2:45-53; PMID: 17012886; http://dx.doi.org/10.4l61/hv. 2.2.2620
Peubez I, Chaudet N, Mignon C, Hild G, Husson S, Courtois V, et al. Antibiotic-free selection in E. coli: new considerations for optimal design and improved production. Microb Cell Fact 2010;9:65; PMID:20822537; http://dx.doi.org/l 0.1186/1475-2859-9-65
Sodoyer R, Courtois V, Peubez I, Mignon C. Antibiotic-Free Selection for Bio-Production: Moving Towards a New "Golden Standard". In: Pana M, ed. Antibiotic Resistant Bacteria - a Continuous Challenge in the New Millennium: InTech, 2012:531-48.
Szpirer CY, Milinkovitch MC. Separate-componentstabilization system for protein and DNA production without the use of antibiotics. Biotechniques 2005;38:775-81; PMID:15945374; http://dx.doi.org/10.2144/05 385RR02
Cherny I, Gazit E. The YefM antitoxin defines a family of natively unfolded proteins: implications as a novel antibacterial target. J Biol Chem 2004;279:8252-61; PMID: 14672926; http://dx.doi.org/10.1074/jbc. M308263200
Guglielmini J, Szpirer C, Milinkovitch MC. Automated discovery and phylogenetic analysis of new toxin-antitoxin systems. BMC Microbiol 2008;8:104; PMID: 18578869; http://dx.doi.org/l 0.1186/1471-2180-8-104
Hayes F, Van Melderen L. Toxins-antitoxins: diversity, evolution and function. Crit Rev Biochem Mol Biol 2011;46:386-408; PMID:21819231; http://dx.doi.org/10.3109/10409238.2011.600437
Hazan R, Sat B, Engelberg-Kulka H. Escherichia coli mazEF-mediated cell death is triggered by various stressful conditions. J Bacteriol 2004;186:3663-9; PMID: 15150257; http://dx.doi.org/10.1128/JB.186.11.3663-3669.2004
Pandey DP, Gerdes K. Toxin-anti toxin loci are highly abundant in free-living but lost from host-associated prokaryotes. Nucleic Acids Res 2005;33:966-76; PMID: 15718296; http://dx.doi.org/10.1093/nar/gki201
Bernard P, Kezdy KE, Van Melderen L, Steyaert J, Wyns L, Pato ML, et al. The F plasmid CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase. J Mol Biol 1993;234:534-41; PMID:8254658; http://dx.doi.org/l 0.1006/jmbi.1993.1609
Salmon MA, Van Melderen L, Bernard P, Couturier M. The antidote and autoregulatory functions of the F plasmid CcdA protein: a genetic and biochemical survey. Mol Gen Genet 1994;244:530-8; PMID:8078480; http://dx.doi.org/l 0.1007/BF00583904
Bernard P Positive selection of recombinant DNA by CcdB. Biotechniques 1996;21:320-3; PMID:8862819
Vandermeulen G, Marie C, Scherman D, Preat V. New Generation of Plasmid Backbones Devoid of Antibiotic Resistance Marker for Gene Therapy Trials. Molecular therapy, the journal of the American Society of Gene Therapy 2011;19:1942-9; http://dx.doi.org/10.1038/mt.2011.182.
Mettenleiter TC. Aujeszkys disease (pseudo rabies) virus: the virus and molecular pathogenesis-state of the art, June 1999. Vet Res 2000;31:99-115; PMID: 10726640; http://dx.doi.org/10.1051/vetres:2000110
Eloit M, Gilardi-Hebenstreit P, Toma B, Perricaudet M. Construction of a defective adenovirus vector expressing the pseudorabies virus glycoprotein gp50 and its use as a live vaccine. J Gen Virol 1990;71:2425-31; PMID:2172456; http://dx.doi.org/10.1099/0022-1317-71-10-2425
Marchioli CC, Yancey RJ Jr., Petrovskis EA, Timmins JG, Post LE. Evaluation of pseudorabies virus glycoprotein gp50 as a vaccine for Aujeszkys disease in mice and swine: expression by vaccinia virus and Chinese hamster ovary cells. J Virol 1987;61:3977-82; PMID:2824827
Van Rooij EM, Rijsewijk FA, Moonen-Leusen HW, Bianchi AT, Rziha HJ. Comparison of different primeboost regimes with DNA and recombinant Orf virus based vaccines expressing glycoprotein D of pseudorabies virus in pigs. Vaccine 2010;28:1808-13; PMID:20018271; http://dx.doi.org/10.1016/j.vaccine.2009.12.004
Wathen LM, Platt KB, Wathen MW, Van Deusen RA, Whetstone CA, Pirtle EC. Production and characterization of monoclonal antibodies directed against pseudorabies virus. Virus Res 1985;4:19-29; PMID: 3002067; http://dx.doi.org/l 0.1016/0168-1702 (85) 90017-6
Hong W, Xiao S, Zhou R, Fang L, He Q, Wu B, et al. Protection induced by intramuscular immunization with DNA vaccines of pseudorabies in mice, rabbits and piglets. Vaccine 2002;20:1205-14; PMID:11803083; http://dx.doi.org/l 0.1016/S0264-410X (01) 00416-9
Yoon HA, Aleyas AG, George JA, Park SO, Han YW, Kang SH, et al. Differential segregation of protective immunity by encoded antigen in DNA vaccine against pseudorabies virus. Immunol Cell Biol 2006;84:502-11; PMID: 16869937; http://dx.doi.org/10.1111/j.1440-1711.2006.01463.x
Van Rooij EM, Haagmans BL, De Visser YE, De Bruin MG, Boersma W, Bianchi AT. Effect of vaccination route and composition of DNA vaccine on the induction of protective immunity against pseudorabies infection in pigs. Vet Immunol Immunopathol 1998;66:113-26; PMID:9860185; http://dx.doi.org/10.1016/S0165-2427 (98) 00186-X
Hwang DY, Lee JB, Kim Song JY, Hyun BH, Song CS, et al. Induction of immune responses to glycoprotein gD of Aujeszkys disease virus with DNA immunization. J Vet Med Sci 2001;63:659-62; PMID: 11459012; http://dx.doi.org/ 10.1292/jvms.63.659
Le Moigne V, Cariolet R, Beven V, Keranflec'h A, Jestin A, Dory D. Electroporation improves the immune response induced by a DNA vaccine against pseudorabies virus glycoprotein B in pigs. Res Vet Sci 2012;93:1032-5; PMID:22051145; http://dx.doi.org/10.1016/j.rvsc.2011.09.020
Gartner T, Romano M, Suin V, Kalai M, Korf H, De Baetselier P, et al. Immunogenicity and protective efficacy of a tuberculosis DNA vaccine co-expressing pro-apoptotic caspase-3. Vaccine 2008;26:1458-70; PMID: 18280621; http://dx.doi.orgZl0.1016/j.vaccine.2007.12.056
Bloquel C, Bejjani R, Bigey P, Bedioui F, Doat M, Ben Ezra D, et al. Plasmid electrotransfer of eye ciliary muscle: principles and therapeutic efficacy using hTNF-alpha soluble receptor in uveitis. FASEB J 2006;20:389-91; PMID:16352651
Van Meerloo J, Kaspers GJ, Cloos J. Cell sensitivity assays: the MTT assay. Methods Mol Biol 2011;731:237-45; PMID:21516412; http:ZZdx.doi.orgZl0. 1007Z978-l-61779-080-5-20
Decker T, Lohmann-Matthes ML. A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J Immunol Methods 1988;115:61-9; PMID:3192948; http://dx.doi.orgZl 0.1016Z0022-1759 (88) 90310-9
Vandermeulen G, Richiardi H, Escriou V, Ni J, Fournier P, Schirrmacher V, et al. Skin-specific promoters for genetic immunisation by DNA electroporation. Vaccine 2009;27:4272-7; PMID: 19450641; http:ZZ dx.doi.orgZl 0.1016Zj.vaccine.2009.05.022
