Effect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts.

Ceusters, Justine; Mouithys-Mickalad, Ange; Franck, Thierry; Deby-Dupont, Ginette; Derochette, Sandrine; Serteyn, Didier

doi:10.1016/j.rvsc.2013.09.004

Article (Scientific journals)

Effect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts.

Ceusters, Justine; Mouithys-Mickalad, Ange; Franck, Thierry et al.

2013 • In Research in Veterinary Science, 95, p. 870-878

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/157412

DOI
10.1016/j.rvsc.2013.09.004

PubMed
24099743

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Keywords :

Cultured primary equine skeletal myoblasts; anoxia/reoxygenation; mitochondria; reactive oxygen species

Abstract :

[en] Horses are outstanding athletes, performing in many different disciplines involving different kinds of efforts and metabolic responses. Depending on exercise intensity, their skeletal muscle oxygenation decreases, and the reperfusion at cessation of the exercise can cause excessive production of free radicals. This study on cultured primary equine myoblasts investigated the effect of different kinds of anoxia/reoxygenation (A/R) on routine respiration, mitochondrial complex I specific activity and free radicals production. Our data revealed that short cycles of A/R caused a decrease of all the parameters, opposite to what a single long period of anoxia did. A preconditioning-like effect could explain our first pattern of results whereas mild uncoupling could be more appropriate for the second one. Anyway, it seems that mitochondrial complex I could play a major role in the regulation of the balance between metabolic and antioxidant protection of the muscular function of athletic horses.

Research Center/Unit :

CORD - Centre de l'Oxygène, Recherche et Développement - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology
Veterinary medicine & animal health

Author, co-author :

Ceusters, Justine ; Université de Liège - ULiège > Clinique des grands animaux (chirurgie)

Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux

Deby-Dupont, Ginette

Derochette, Sandrine ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux

Language :

English

Title :

Effect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts.

Publication date :

December 2013

Journal title :

Research in Veterinary Science

ISSN :

0034-5288

eISSN :

1532-2661

Publisher :

British Veterinary Association, London, United Kingdom

Volume :

Pages :

870-878

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture

Available on ORBi :

since 21 October 2013

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