Overexpression of CD9 in human breast cancer cells promotes the development of bone metastases.

Aged; Aged, 80 and over; Animals; Antigens, CD9/biosynthesis; Bone Neoplasms/metabolism/secondary; Breast Neoplasms/metabolism/pathology; Cell Line, Tumor; Female; Humans; Immunoblotting; Mice; Mice, Inbred BALB C; Mice, Nude; Middle Aged; Tumor Markers, Biological/biosynthesis; Up-Regulation

Abstract :

[en] BACKGROUND: Bone is a preferred target for circulating metastatic breast cancer cells. We found that the CD9 protein was up-regulated in the B02 osteotropic cell line, derived from the aggressive parental MDA-MB-231 breast cancer cell line. Here, we investigated the putative relationship between CD9 expression and the osteotropic phenotype. MATERIALS AND METHODS: Overexpression of CD9 was analyzed by immunoblotting in different cell lines. Immunohistochemistry was used to assess CD9 expression in primary tumors and metastatic lesions. In vivo experiments were conducted in mice using a monoclonal antibody against CD9. RESULTS: CD9 overexpression was confirmed in osteotropic cells. CD9 was significantly overexpressed in bone metastases versus primary tumors and visceral metastatic lesions. Finally, in vivo experiments showed that an antibody against CD9 delays homing of B02 cells in bone marrow, slowing down bone destruction. CONCLUSION: Our study reveals a potential implication of CD9 in the formation of bony metastases from breast cancer cells.

Research Center/Unit :

Giga-Cancer - ULiège

Disciplines :

Oncology
Biochemistry, biophysics & molecular biology

Author, co-author :

Kischel, Philippe

Bellahcene, Akeila ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases

Deux, Blandine

Lamour, Virginie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases

Dobson, Rowan

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Clezardin, Philippe

Castronovo, Vincenzo ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire

Language :

English

Title :

Overexpression of CD9 in human breast cancer cells promotes the development of bone metastases.

Publication date :

2012

Journal title :

Anticancer Research

ISSN :

0250-7005

eISSN :

1791-7530

Publisher :

International Institute of Anticancer Research, Greece

Volume :

Issue :

Pages :

5211-20

Peer reviewed :

Peer Reviewed verified by ORBi

European Projects :

FP7 - 201342 - ADAMANT - ANTIBODY DERIVATIVES AS MOLECULAR AGENTS FOR NEOPLASTIC TARGETING

Funders :

EU FP6 STROMA
EU FP7 ADAMANT
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Fondation Léon Fredericq
CE - Commission Européenne

Available on ORBi :

since 06 September 2013

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