Crystal structure of a cold-adapted class C beta-lactamase.

Amino Acid Sequence; Cold Temperature; Crystallography, X-Ray; Enzyme Stability/drug effects; Kinetics; Models, Molecular; Molecular Sequence Data; Protein Structure, Tertiary; Pseudomonas fluorescens/enzymology; Sequence Alignment; Structural Homology, Protein; Urea/pharmacology; beta-Lactamases/chemistry/classification/metabolism

Abstract :

[en] In this study, the crystal structure of a class C beta-lactamase from a psychrophilic organism, Pseudomonas fluorescens, has been refined to 2.2 A resolution. It is one of the few solved crystal structures of psychrophilic proteins. The structure was compared with those of homologous mesophilic enzymes and of another, modeled, psychrophilic protein. The elucidation of the 3D structure of this enzyme provides additional insights into the features involved in cold adaptation. Structure comparison of the psychrophilic and mesophilic beta-lactamases shows that electrostatics seems to play a major role in low-temperature adaptation, with a lower total number of ionic interactions for cold enzymes. The psychrophilic enzymes are also characterized by a decreased number of hydrogen bonds, a lower content of prolines, and a lower percentage of arginines in comparison with lysines. All these features make the structure more flexible so that the enzyme can behave as an efficient catalyst at low temperatures.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Michaux, Catherine

Massant, Jan

Kerff, Frédéric ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Frère, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Vandenberghe, Isabel

Samyn, Bart

Pierrard, Annick ; Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation

Feller, Georges ; Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie

Charlier, Paulette ; Université de Liège - ULiège > Département des sciences de la vie > Cristallographie des macromolécules biologiques

Van Beeumen, Jozef

Wouters, Johan

Language :

English

Title :

Crystal structure of a cold-adapted class C beta-lactamase.

Publication date :

2008

Journal title :

FEBS Journal

ISSN :

1742-464X

eISSN :

1742-4658

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

275

Issue :

Pages :

1687-97

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 January 2010

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