Article (Scientific journals)
Crystal structure of a cold-adapted class C beta-lactamase.
Michaux, Catherine; Massant, Jan; Kerff, Frédéric et al.
2008In FEBS Journal, 275 (8), p. 1687-97
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Keywords :
Amino Acid Sequence; Cold Temperature; Crystallography, X-Ray; Enzyme Stability/drug effects; Kinetics; Models, Molecular; Molecular Sequence Data; Protein Structure, Tertiary; Pseudomonas fluorescens/enzymology; Sequence Alignment; Structural Homology, Protein; Urea/pharmacology; beta-Lactamases/chemistry/classification/metabolism
Abstract :
[en] In this study, the crystal structure of a class C beta-lactamase from a psychrophilic organism, Pseudomonas fluorescens, has been refined to 2.2 A resolution. It is one of the few solved crystal structures of psychrophilic proteins. The structure was compared with those of homologous mesophilic enzymes and of another, modeled, psychrophilic protein. The elucidation of the 3D structure of this enzyme provides additional insights into the features involved in cold adaptation. Structure comparison of the psychrophilic and mesophilic beta-lactamases shows that electrostatics seems to play a major role in low-temperature adaptation, with a lower total number of ionic interactions for cold enzymes. The psychrophilic enzymes are also characterized by a decreased number of hydrogen bonds, a lower content of prolines, and a lower percentage of arginines in comparison with lysines. All these features make the structure more flexible so that the enzyme can behave as an efficient catalyst at low temperatures.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Michaux, Catherine
Massant, Jan
Kerff, Frédéric  ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Frère, Jean-Marie ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Docquier, Jean-Denis ;  Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Vandenberghe, Isabel
Samyn, Bart
Pierrard, Annick ;  Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation
Feller, Georges ;  Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie
Charlier, Paulette ;  Université de Liège - ULiège > Département des sciences de la vie > Cristallographie des macromolécules biologiques
Van Beeumen, Jozef
Wouters, Johan
Language :
English
Title :
Crystal structure of a cold-adapted class C beta-lactamase.
Publication date :
2008
Journal title :
FEBS Journal
ISSN :
1742-464X
eISSN :
1742-4658
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
275
Issue :
8
Pages :
1687-97
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 January 2010

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