[en] BACKGROUND: Whereas multi-species biofilm reactors are commonly used for the treatment of liquid and solid wastes,
new strategies are progressing for the development of single species biofilm for the production of high-value metabolites.
Technically, this new concept relies on the design of bioreactors able to promote biofilm formation and on the identification of
the key physico-chemical parameters involved in biofilm formation.
RESULTS: An experimental setting comprising a liquid continuously recirculated on a metal structured packing has been used
to promote Bacillus subtilis GA1 biofilm formation. The colonization of the packing has been visualized non-invasively by
X-ray tomography. This analysis revealed an uneven, conical, distribution of the biofilm inside the packing. Compared with a
submerged culture carried out in a stirred tank reactor, significant modification of the lipopeptide profile has been observed in
the biofilm reactorwith the disappearance of fengycin and iturin fractions and an increase of the surfactin fraction. In addition,
considering the biofilm reactor design, no foam formation has been observed during the culture.
CONCLUSIONS: The configuration of this biofilm reactor set-up allows for a higher surfactin production by comparison with a
submerged culture while avoiding foam formation. Additionally, scale-up could easily be performed by increasing the number
of packing elements.
Disciplines :
Biotechnology
Author, co-author :
Zune, Quentin ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Soyeurt, Delphine
Toye, Dominique ; Université de Liège - ULiège > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques
Ongena, Marc ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Delvigne, Frank ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Language :
English
Title :
High-energy X-ray tomography analysis of a metal packing biofilm reactor for the production of lipopeptides by Bacillus subtilis
Publication date :
2014
Journal title :
Journal of Chemical Technology and Biotechnology
ISSN :
0268-2575
Publisher :
John Wiley & Sons Ltd., Chichester, United Kingdom
Volume :
89
Pages :
382-390
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Rosche B, Li XZ, Hauer B, Schmid A and Buehler K, Microbial biofilms: a concept for industrial catalysis? Trends Biotechnol 27:636-643 (2009).
Qureschi N, Annous BA, Ezeji TC, Karcher P and Maddox IS, Biofilm reactors for industrial bioconversion processes: employing potential of enhanced reaction rates. Microbial Cell Factories 4:1-21 (2006).
Tsoligkas AN, Winn M, Bowen J, Overton TW, Simmons MJH and Goss RJM, Engineering biofilms for biocatalysis. Chem Bio Chem 12:1391-1395 (2011).
Setyawati MI, Chien LJ and Lee CK, Self-immobilized recombinant acetobacter xylinum for biotransformation. Biochem Eng J 43:78-84 (2009).
Gross R, Lang K, Bühler K and Schmid A, Characterization of a biofilm membrane reactor and its prospects for fine chemical synthesis. Biotechnol Bioeng 105:705-717 (2009).
Li XZ, Webb JS, Kjelleberg S and Rosche B, Enhanced benzaldehyde tolerance in Zymomonas mobilis biofilms and the potential of biofilm applications in fine-chemical production. Appl Environ Microbiol 72:1639-1644 (2006).
Gross R, Hauer B, Otto K and Schmid A, Microbial biofilms: new catalysts for maximizing productivity of long-term biotransformations. Biotechnol Bioeng 98:1123-1134 (2007).
Renslow R, Lewandowski Z and Beyenal H, Biofilm image reconstruction for assessing structural parameters. Biotechnol Bioeng 108:1383-1394 (2011).
Tolker-Nielsen T and Sternberg C, Growing and analyzing biofilms in flow chambers. Curr Protocols Microbiol 21:1B.2.1-B.2.17 (2011).
Pamp SJ, Sternberg C and Tolker-Nielsen T, Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy. Cytometry Part A 75A:90-103 (2009).
Davit Y, Iltis G, Debenest G, Veran-Tissoires S, Wildenschild D, Gerino M and Quintard M, Imaging biofilm in porous media using X-ray computed microtomography. J Microsc 242:15-25 (2011).
Haisch C and Niessner R, Visualtisation of transient processes in biofilms by optical coherence tomography. Water Res 41:2467-2472 (2007).
Wagner M, Taherzadeh D, Haisch C and Horn H, Investigation of the mesoscale structure and volumetric features of biofilms using optical coherence tomography. Biotechnol Bioeng 107:844-853 (2010).
Morgenroth E and Milferstedt K, Biofilm engineering: linking biofilm development at different length and time scales. Rev Environ Sci Biotechnol 8:203-208 (2009).
Shaligram NS and Singhal RS, Surfactin - a review on biosynthesis, fermentation, purification and applications. Food Technol Biotechnol 48:119-134 (2010).
Viva A, Aferka S, Brunazzi E, Marchot P, Crine M and Toye D, Processing X-ray tomographic images: a procedure adapted for the analysis of phase distribution in MellapakPlus 752.Y and Katapak-SP packings. Flow Measurement Instrumentation 22:279-290 (2011).
Aferka S, Viva A, Brunazzi E, Marchot P, Crine M and Toye D, Tomographic measurement of liquid hold-up and effective interfacial area distributions in a column packed with high performance structured packings. Chem Eng Sci 66:3413-3422 (2011).
Nihorimbere V, Cawoy H, Seyer A, Brunelle A, Thonart P and Ongena M, Impact of rhizosphere factors on cyclic lipopeptide signature from the plant beneficial strain Bacillus amyloliquefaciens S499. FEMS Microbiol Ecol 79:176-191 (2011).
Marvasi M, Visscher PT and Martinez LC Exopolymeric substances (EPS) from Bacillus subtilis : polymers and genes encoding their synthesis. FEMS Microbiol Lett 313:1-9 (2010).
Lopez D, Fischbach MA, Chu F, Losick R and Kolter R, Structurally diverse natural products that cause potassium leakage trigger multicellularity in Bacillus subtilis. PNAS 106:280-285 (2008).
Jacques P, Hbid C, Destain J, Razafindralambo H, Paquot M, De Pauw E and Thonart P, Optimization of biosurfactant lipopetide production from Bacillus subtilis S499 by Plackett-Burman design. Appl Biochem Biotechnol 77-79:223-233 (1999).
Chtioui O, Dimitrov K, Gancel F and Nikov I, Biosurfactant production by immobilized cells of Bacillus subtilis ATCC 21332 and their recovery by pertraction. Process Biochem 45:1795-1799 (2010).
Gancel F, Montastruc L, Liu T, Zhao L and Nikov I, Lipopeptides overproduction by cell immobilization on iron-enriched light polymer particles. Process Biochem 44:975-978 (2009).