Real-time monitoring of cell viability and cell density on the basis of a three dimensional optical reflectance method (3D-ORM): investigation of the effect of sub-lethal and lethal injuries
[en] Cell density and cell viability have been followed on-line by using a three-dimensional optical reflectance method (3D-ORM) probe. This method has allowed to highlight the differences between a well-mixed and a scale-down bioreactor configured in order to reproduce mixing deficiencies during a fed-batch culture of E. coli. These differences have been observed both for the obscuration factor (OBF) and the coincidence probability (COP) delivered by the probe. These parameters are correlated to flow cytometry measurement based on the PI-uptake test and cell density based on optical density measurement. This first set of results has pointed out the fact that the 3D-ORM probe is sensitive to sub-lethal injuries encountered by microbial cells in process-related conditions. The effect of lethal injuries has been further investigated on the basis of additional experiments involving heat stress and a sharp increase of the OBF has been observed indicating that cells are effectively injured by the increase of temperature. However, further improvement of the probe are needed in order to give access to single-cell measurements.
Disciplines :
Biotechnology
Author, co-author :
Brognaux, Alison ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Bugge, Jörg
Schwartz, Friedel
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Telek, Samuel ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Delvigne, Frank ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Language :
English
Title :
Real-time monitoring of cell viability and cell density on the basis of a three dimensional optical reflectance method (3D-ORM): investigation of the effect of sub-lethal and lethal injuries
Publication date :
2013
Journal title :
Journal of Industrial Microbiology and Biotechnology
ISSN :
1367-5435
eISSN :
1476-5535
Publisher :
Springer, Berlin, Germany
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
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