[en] Homeodomain-containing proteins are transcription factors regulating the coordinated expression of multiple target genes involved in development, differentiation and cellular transformation. In this study, we demonstrated that HOXB7, one member of this family, behaved as a transactivator in breast cancer cells. Deletion of either the HOXB7 N-terminal domain or the C-terminal acidic tail abolished this transcriptional effect, suggesting a combination of distinct functional transactivating domains. HOXB7 physically interacted both in vitro and in vivo with the coactivator CREB-binding protein (CBP). This interaction led to an enhanced transactivating potential and required the N-terminal of HOXB7 as well as two domains located at the C-terminal part of CBP. Moreover, trichostatin A, a deacetylase inhibitor, strongly enhanced the transcriptional properties of HOXB7. Our data therefore indicate that HOX proteins can directly interact with CBP and that acetylation/deacetylation may regulate their transcriptional properties.
Centre/Unité de recherche :
Giga-Signal Transduction - ULiège
Disciplines :
Biochimie, biophysique & biologie moléculaire
Auteur, co-auteur :
Chariot, Alain ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Van Lint, Carine
Chapelier, Muriel
Gielen, Jacques
Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Bours, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Génétique générale et humaine
Langue du document :
Anglais
Titre :
CBP and histone deacetylase inhibition enhance the transactivation potential of the HOXB7 homeodomain-containing protein
Date de publication/diffusion :
1999
Titre du périodique :
Oncogene
ISSN :
0950-9232
eISSN :
1476-5594
Maison d'édition :
Nature, Basingstoke, Royaume-Uni
Volume/Tome :
18
Pagination :
4007-4012
Peer reviewed :
Peer reviewed vérifié par ORBi
Organisme subsidiant :
F.R.S.-FNRS - Fonds de la Recherche Scientifique Télévie
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