Article (Scientific journals)
Effect of extraction conditions on the yied and purity of ulvan extracted from Ulva lactuca
Yaich, Hela; Garna, Haikel; Besbes, Souhail et al.
2013In Food Hydrocolloids, p. 375-382
Peer Reviewed verified by ORBi
 

Files


Full Text
Effect of extraction conditions on the yied.pdf
Publisher postprint (4.39 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Ulvan; Ulvan lactuca; Extraction conditions; Alcohol; Yields; Polysaccharide characterization
Abstract :
[en] A study of the influence of extraction conditions (pH: 1.5 and 2; temperature: 80 °C and 90 °C; extraction time: 1-3 h), on the yield, chemical composition and purity of the sulphated cell wall polysaccharides ulvan, extracted from the green seaweed Ulva lactuca and precipitated by alcohol is carried out. The alcohol precipitate yields varied from 21.68% to 32.67% (%dw/dw) depending on the pH. At pH 2, the alcohol precipitate yields and the uronic acid recovery from extract juice are higher than those obtained at pH 1.5. Other compounds than ulvan such as cellulose, hemicellulose, proteins and ash are solubilized from the cell walls of Ulva lactuca at both pH, and they are precipitated with alcohol. The alcohol precipitate obtained from different extraction conditions has high uronic acid (20.37%-23.60%) and neutral sugar content (20.09%-29.12%), especially when the conditions (pH, temperature) are drastic. It contains rhamnose (13.35%-15.59%), glucose (2.90%-10.97%), and xylose (2.36%-2.73%). A decrease in the molecular weight of ulvan was observed at acid pH, and for long extraction times. The presence of proteins (1.94%-2.32%) and inorganic material (33.36%-47.15%) in alcohol precipitate prove the lower purity of ulvan extracted and shows that ulvan precipitation with ethanol is not specific. © 2012 Elsevier Ltd.
Disciplines :
Food science
Author, co-author :
Yaich, Hela
Garna, Haikel
Besbes, Souhail
Paquot, Michel 
Blecker, Christophe ;  Université de Liège - ULiège > Chimie et bio-industries > Science des alim. et formul.
Attia, Hamadi
Language :
English
Title :
Effect of extraction conditions on the yied and purity of ulvan extracted from Ulva lactuca
Publication date :
2013
Journal title :
Food Hydrocolloids
ISSN :
0268-005X
eISSN :
1873-7137
Publisher :
Elsevier Science
Pages :
375-382
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 February 2013

Statistics


Number of views
253 (9 by ULiège)
Number of downloads
95 (2 by ULiège)

OpenCitations
 
0

Bibliography


Similar publications



Contact ORBi