Construction of a medium-density horse gene map

[en] A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the INRA and the CHORI-241 equine BAC libraries by polymerase chain reaction and by filter hybridization respectively. Two hundred and thirty-seven BACs containing equine gene orthologues, confirmed by sequencing, were isolated. The BACs were localized to horse chromosomes by fluorescent in situ hybridization (FISH). Overall, 165 genes were assigned to the equine genomic map by radiation hybrid (RH) (using an equine RH5000 panel) and/or by FISH mapping. A comparison of localizations of 713 genes mapped on the horse genome and on the human genome revealed 59 homologous seg- ments and 131 conserved segments. Two of these homologies (ECA27/HSA8 and ECA12p/ HSA11p) had not been previously identified. An enhanced resolution of conserved and rearranged chromosomal segments presented in this study provides clarification of chromosome evolution history.

Disciplines :

Genetics & genetic processes

Author, co-author :

Perrocheau, M; Institut Scientifique de Recherche Agronomique - INRA > Département de Génetique animale

Chadi, S

Mata, X

Decaunes, P

Raudsepp, T

Durkin, Keith ; Texas A&M University > Department of Veterinary Anatomy & Public Health > College of Veterinary Medicine

Incarnato, D

Iannuzzi, L

Lear, T. L.

Hirota, K

More authors (6 more)

Language :

English

Title :

Construction of a medium-density horse gene map

Publication date :

2006

Journal title :

Animal Genetics

ISSN :

0268-9146

eISSN :

1365-2052

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 11 January 2013

Statistics

Number of views

16 (1 by ULiège)

Number of downloads

1 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Bourque G. Pevzner P.A. 2002 Genome-scale evolution: reconstructing gene orders in the ancestral species. Genome Research 12 26 36.
Cai W.W. Reneker J. Chow C.W. Vaishnav M. Bradley A. 1998 An anchored framework BAC map of mouse chromosome 11 assembled using multiplex oligonucleotide hybridization. Genomics 54 387 97.
Chowdhary B.P. Raudsepp T. Fronicke L. Scherthan H. 1998 Emerging patterns of comparative genome organization in some mammalian species as revealed by zoo-FISH. Genome Research 8 577 89.
Chowdhary B.P. Raudsepp T. Honeycutt D. Owens E.K. Piumi F. Guerin G. Matise T.C. Kata S.R. Womack J.E. Skow L.C. 2002 Construction of a 5000(rad) whole-genome radiation hybrid panel in the horse and generation of a comprehensive and comparative map for ECA11. Mammalian Genome 13 89 94.
Chowdhary B.P. Raudsepp T. Kata S.R. et al. 2003 The first-generation whole-genome radiation hybrid map in the horse identifies conserved segments in human and mouse genomes. Genome Research 13 742 51.
Farber C.R. Medrano J.F. 2004 Identification of putative homology between horse microsatellite flanking sequences and cross-species ESTs, mRNAs and genomic sequences. Animal Genetics 35 28 33.
Guérin G. Bailey E. Bernoco D. et al. 2003 The second generation of the International Equine Gene Mapping Workshop half-sibling linkage map. Animal Genetics 34 161 8.
Gustafson-Seabury A. Raudsepp T. Goh G. Kata S.R. Wagner M.L. Tozaki T. Mickelson J.R. Womack J.E. Skow L.C. Chowdhary B.P. 2005 High-resolution RH map of horse chromosome 22 reveals a putative ancestral vertebrate chromosome. Genomics 85 188 200.
Hirota K. Tozaki T. Mashima S. Miura N. 2001 Cytogenetic assignment and genetic characterization of the horse microsatellites, TKY4-18, TKY20, TKY22-24, TKY30-41 derived from a cosmid library. Animal Genetics 32 160 7.
ISCHN Bowling A.T. Breen M. Chowdhary B.P. Hirota K. Lear T. Millon L.V. Leon FAPd Raudsepp T. Stranzinger G. 1997 International system for cytogenetic nomenclature of the domestic Horse. Report of the Third International Committee for the Standardization of the Domestic Horse Karyotype. Davis, CA, USA, 1996. Chromosome Research 5 433 43.
Laurent P. Elduque C. Hayes H. Saunier K. Eggen A. Leveziel H. 2000 Assignment of 60 human ESTs in cattle. Mammalian Genome 11 748 54.
Lear T.L. Brandon R. Piumi F. Terry R.R. Guerin G. Thomas S. Bailey E. 2001 Mapping of 31 horse genes in BACs by FISH. Chromosome Research 9 261 2.
Lee E.J. Raudsepp T. Kata S.R. Adelson D. Womack J.E. Skow L.C. Chowdhary B.P. 2004 A 1.4-Mb interval RH map of horse chromosome 17 provides detailed comparison with human and mouse homologues. Genomics 83 203 15.
Lindgren G. Breen M. Godard S. et al. 2001 Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis. Chromosome Research 9 53 59.
Mariat D. Oustry-Vaiman A. Cribiu E.P. Raudsepp T. Chowdhary B. Guérin G. 2001 Isolation, characterization and FISH assignments of horse BAC clones containing type I and II markers. Cytogenetics and Cell Genetics 92 144 8.
Milenkovic D. Oustry-Vaiman A. Lear T.L. Billault A. Mariat D. Piumi F. Schibler L. Cribiu E. Guerin G. 2002 Cytogenetic localization of 136 genes in the horse: Comparative mapping with the human genome. Mammalian Genome 13 524 34.
Muller C. Denis M. Gentzbittel L. Faraut T. 2004 The Iccare Web server: an attempt to merge sequence and mapping information for plant and animal species. Nucleic Acids Research 32 W429 34.
Murphy W.J. Stanyon R. O'Brien S.J. 2001 Evolution of mammalian genome organization inferred from comparative gene mapping. Genome Biology 2 REVIEWS0005.
Murphy W.J. Larkin D.M. Everts-van der Wind A. et al. 2005 Dynamics of mammalian chromosome evolution inferred from multispecies comparative maps. Science 22 613 7.
Penedo M.C.T. Millon L.V. Bernoco D. et al. 2005 International equine gene mapping workshop report: A comprehensive linkage map constructed with data from new markers and by merging four mapping resources. Cytogenetic and Genome Research 111 5 15.q16
Perrocheau M. Boutreux V. Chadi-Taourit S. Di Meo G.P. Perucatti A. Incarnato D. Cribiu E.P. Guérin G. Iannuzzi L. 2005 Equine FISH mapping of 36 genes known to locate on human chromosome ends. Cytogenetic and Genome Research 111 46 50.
Pevzner P. Tesler G. 2003 Genome rearrangements in mammalian evolution: Lessons from human and mouse genomes. Genome Research 13 37 45.
Raudsepp T. Fronicke L. Scherthan H. Gustavsson I. Chowdhary B.P. 1996 Zoo-FISH delineates conserved chromosomal segments in horse and man. Chromosome Research 4 218 25.
Raudsepp T. Kata S.R. Piumi F. Swinburne J. Womack J.E. Skow L.C. Chowdhary B.P. 2002 Conservation of gene order between horse and human X chromosomes as evidenced through radiation hybrid mapping. Genomics 79 451 7.
Raudsepp T. Lee E.J. Kata S.R. Brinkmeyer C. Mickelson J.R. Skow L.C. Womack J.E. Chowdhary B.P. 2004 Exceptional conservation of horse-human gene order on X chromosome revealed by high-resolution radiation hybrid mapping. Proceedings of the National Academy of Sciences of the United States of America 101 2386 91.
Richard F. Lombard M. Dutrillaux B. 2003 Reconstruction of the ancestral karyotype of eutherian mammals. Chromosome Research 11 605 18.
Sambrook J. Fritsch E.F. Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd ed. Cold Spring Harbor Press Cold Spring Harbor, NY.
Shiue Y.L. Bickel L.A. Caetano A.R. et al. 1999 A synteny map of the horse genome comprised of 240 microsatellite and RAPD markers. Animal Genetics 30 1 9.