Airway inflammation in cadmium-exposed rats is associated with pulmonary oxidative stress and emphysema

[en] The aim of this study was to test the hypothesis that pulmonary inflammation and emphysema induced by cadmium (Cd) inhalation are associated with pulmonary oxidative stress. Two groups of Sprague Dawley rats were used: one vehicle-exposed group undergoing inhalation of NaCl (0.9%, n = 24) and one Cd-exposed group undergoing inhalation of CdCl(2) (0.1%, n = 24). The animals in the vehicle-and Cd-exposed groups were divided into 4 subgroups (n = 6 per group), which underwent either a single exposure (D2) of 1H or repeated exposures 3 times/week for 1H for a period of 3 weeks (3W), 5 weeks (5W) or 5 weeks followed by 2 weeks without exposure (5W + 2). At sacrifice, the left lung was fixed for histomorphometric analysis (median inter-wall distance, MIWD), whilst bronchoalveolar lavage fluid (BALF) was collected from the right lung. Cytological analysis of BALF was performed and BALF was analysed for oxidant markers 8-iso-PGF(2a), uric acid (UA), reduced (AA) and oxidised ascorbic acid (DHA) and reduced (GSH) and oxidised glutathione (GSSG). Cd-exposure induced a significant increase of BALF macrophages and neutrophils. 8-iso-PGF(2a), UA, GSH and GSSG were significantly increased at D2. At 5W and 5W + 2, AA and GSH were significantly lower in Cd-exposed rats, indicating antioxidant depletion. MIWD significantly increased in all repeatedly Cd-exposed groups, suggesting development of pulmonary emphysema. 8-iso-PGF(2a) and UA were positively correlated with macrophage and neutrophil counts. GSH, GSSG and 8-iso-PGF(2a) were negatively correlated with MIWD, indicating that Cd-induced emphysema could be associated with pulmonary oxidative stress.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Kirschvink, Nathalie

Martin, N.

Fievez, Laurence ; Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie et biologie moléculaire

Smith, Nicolas

Marlin, David

Gustin, Pascal ; Université de Liège - ULiège > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie

Language :

English

Title :

Airway inflammation in cadmium-exposed rats is associated with pulmonary oxidative stress and emphysema

Publication date :

2006

Journal title :

Free Radical Research

ISSN :

1071-5762

eISSN :

1029-2470

Publisher :

Taylor & Francis Ltd, Abingdon, United Kingdom

Volume :

Issue :

Pages :

241-250

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 04 June 2009

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