Effect of myeloperoxidase and anoxia/reoxygenation on mitochondrial respiratory function of cultured primary equine skeletal myoblasts.

Ceusters, Justine; Mouithys-Mickalad, Ange; Franck, Thierry; Derochette, Sandrine; Vanderplasschen, Alain; Deby-Dupont, Ginette; Serteyn, Didier

doi:10.1016/j.mito.2012.12.004

Article (Scientific journals)

Effect of myeloperoxidase and anoxia/reoxygenation on mitochondrial respiratory function of cultured primary equine skeletal myoblasts.

Ceusters, Justine; Mouithys-Mickalad, Ange; Franck, Thierry et al.

2013 • In Mitochondrion, 13 (5)

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/137524

DOI
10.1016/j.mito.2012.12.004

PubMed
23268199

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Keywords :

Myeloperoxidase; Primary equine skeletal myoblasts; Anoxia/reoxygenation; Mitochondria; High resolution oxymetry; Reactive oxygen and nitrogen species

Abstract :

[en] Horses are particularly sensitive to excessive inflammatory reaction where myeloperoxidase, a marker of inflammation, may contribute to mitochondrial dysfunctions. This study investigated the interaction between myeloperoxidase and cultured primary equine skeletal myoblasts, particularly its effect on mitochondrial respiration combined or not with anoxia followed by reoxygenation (AR). We showed that active myeloperoxidase entered into the cells, interacted with mitochondria and decreased routine and maximal respirations. When combined with AR, myeloperoxidase caused a further decrease of these respiratory parameters while the leak increased. Our results indicate that myeloperoxidase amplifies the mitochondrial damages initiated by AR phenomenon and alters the mitochondrial function.

Research Center/Unit :

CORD - Centre de l'Oxygène, Recherche et Développement - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology
Veterinary medicine & animal health

Author, co-author :

Ceusters, Justine ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux

Derochette, Sandrine ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Vanderplasschen, Alain ; Université de Liège - ULiège > Immunologie et vaccinologie

Deby-Dupont, Ginette

Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux

Language :

English

Title :

Effect of myeloperoxidase and anoxia/reoxygenation on mitochondrial respiratory function of cultured primary equine skeletal myoblasts.

Publication date :

2013

Journal title :

Mitochondrion

ISSN :

1567-7249

eISSN :

1872-8278

Publisher :

Elsevier Science

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture

Available on ORBi :

since 07 January 2013

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