[en] Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely CHCA (alpha-cyano-4-hydroxycinnamic acid), in combination with alpha-cyclodextrine, allows efficient ionization of poorly soluble lignin materials and suppression of matrix-related ions background. Analysis of low-mass fragments ions (m/z 100-600) in the positive ion mode offers a “fingerprint” of starting lignins that could be a fine strategy to qualitatively identify principal interunit linkages between phenylpropanoid units. The molecular weights of lignins are estimated using size exclusion chromatography and compared to MALDI-TOF-MS profiles. Miscanthus (Miscanthus x giganteus) and Switchgrass (Panicum Virgatum L.) lignins, recovered after a formic acid/acetic acid/water process or aqueous ammonia soaking, are selected as benchmarks for this study.
Disciplines :
Chemistry
Author, co-author :
Richel, Aurore ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Vanderghem, Caroline ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Simon, Mathilde ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Wathelet, Bernard ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Paquot, Michel ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Language :
English
Title :
Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for second-generation lignin analysis
Publication date :
2012
Journal title :
Analytical Chemistry Insights
eISSN :
1177-3901
Publisher :
Libertas Academica Ltd.
Volume :
7
Pages :
79-89
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
TECHNOSE. Bioraffinerie végétale. Chimie et technologie des structures osidiques
Funders :
DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE]
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