The essential cell division protein FtsN interacts with the murein (peptidoglycan) synthase PBP1B in Escherichia coli

[en] Bacterial cell division requires the coordinated action of cell division proteins and murein (peptidoglycan) synthases. Interactions involving the essential cell division protein FtsN and murein synthases were studied by affinity chromatography with membrane fraction. The murein synthases PBP1A, PBP1B, and PBP3 had an affinity to immobilized FtsN. FtsN and PBP3, but not PBP1A, showed an affinity to immobilized PBP1B. The direct interaction between FtsN and PBP1B was confirmed by pulldown experiments and surface plasmon resonance. The interaction was also detected by bacterial two-hybrid analysis. FtsN and PBP1B could be cross-linked in intact cells of the wild type and in cells depleted of PBP3 or FtsW. FtsN stimulated the in vitro murein synthesis activities of PBP1B. Thus, FtsN could have a role in controlling or modulating the activity of PBP1B during cell division in Escherichia coli.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Muller, P.

Ewers, C.

Bertsche, U.

Anstett, M.

Kallis, T.

Breukink, E.

Fraipont, Claudine ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Terrak, Mohammed ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Nguyen-Distèche, Martine ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Vollmer, W.

Language :

English

Title :

The essential cell division protein FtsN interacts with the murein (peptidoglycan) synthase PBP1B in Escherichia coli

Publication date :

14 December 2007

Journal title :

Journal of Biological Chemistry

ISSN :

0021-9258

eISSN :

1083-351X

Publisher :

Amer Soc Biochemistry Molecular Biology Inc, Bethesda, United States - Maryland

Volume :

282

Issue :

Pages :

36394-36402

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1426562&blobtype=pdf

Available on ORBi :

since 25 May 2009

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150

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