Article (Scientific journals)
Characterization of the bifunctional glycosyltransferase/acyltransferase penicillin-binding protein 4 of Listeria monocytogenes
Zawadzka-Skomial, J.; Markiewicz, Z.; Nguyen-Distèche, Martine et al.
2006In Journal of Bacteriology, 188 (5), p. 1875-1881
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Abstract :
[en] Multimodular penicillin-binding proteins (PBPs) are essential enzymes responsible for bacterial cell wall peptidoglycan (PG) assembly. Their glycosyltransferase activity catalyzes glycan chain elongation from lipid II substrate (undecaprenyl-pyrophosphoryi-N-acetylglucosamine-N-acetylmuramic acid-pentapeptide), and their transpeptidase activity catalyzes cross-linking between peptides carried by two adjacent glycan chains. Listeria monocytogenes is a food-borne pathogen which exerts its virulence through secreted and cell wall PG-associated virulence factors. This bacterium has five PBPs, including two bifunctional glycosyltransferase/transpeptidase class A PBPs, namely, PBP1 and PBP4. We have expressed and purified the latter and have shown that it binds penicillin and catalyzes in vitro glycan chain polymerization with an efficiency of 1,400 M-1 s(-1) from Escherichia coli lipid II substrate. PBP4 also catalyzes the aminolysis (D-Ala as acceptor) and hydrolysis of the thiolester donor substrate benzoyl-Gly-thioglycolate, indicating that PBP4 possesses both transpeptidase and carboxyeptidase activities. Disruption of the gene lmo2229 encoding PBP4 in L. monocytogenes EGD did not. p have any significant effect on growth rate, peptidoglycan composition, cell morphology, or sensitivity to beta-lactam antibiotics but did increase the resistance of the mutant to moenomycin.
Disciplines :
Microbiology
Author, co-author :
Zawadzka-Skomial, J.
Markiewicz, Z.
Nguyen-Distèche, Martine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Devreese, B.
Frère, Jean-Marie ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Terrak, Mohammed  ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Characterization of the bifunctional glycosyltransferase/acyltransferase penicillin-binding protein 4 of Listeria monocytogenes
Publication date :
March 2006
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
Amer Soc Microbiology, Washington, United States - Washington
Volume :
188
Issue :
5
Pages :
1875-1881
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 May 2009

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