[en] The method presented in this paper should prove useful in assessing the effectiveness of HACCP plans developed in slaughterhouses. Samples were collected by swabbing well-defined areas of pork and beef carcasses with sterile gauze. Between 160 and 420 half-carcasses were swabbed in each of nine pork or beef slaughterhouses. Swabs from five carcasses were placed in the same sterile Stomacher bag, constituting a single composite sample. Standard or validated analytical methods were used to isolate and characterize four foodborne pathogens. Salmonella spp., Listeria monocytogenes, Campylobacter spp., and verocytotoxin-producing E. coli were detected, respectively, in 27, 2, 2, and 14% of the pork samples and 0, 22, 10, and 5% of the beef samples. Of the 10 samples positive for E. coli O157, only one yielded an isolate confirmed to be enterohemorrhagic. Since Salmonella spp. appear as the main contaminant port (27%) and L. monocytogenes as the main containment of beef (22%), any slaughterhouse sampling plan should include testing for the former in the case of pork carcasses and for the latter in the case of beef carcasses. One should also test regularly for the presence of E. coli O157 and Campylobacter spp. in pork and beef abattoirs. The method presented here is an easy way to assess the contamination rate of carcasses at the end of the slaughtering process.
Disciplines :
Microbiology Food science
Author, co-author :
Korsak Koulagenko, Nicolas ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Ghafir, Yasmine; Université de Liège - ULiège > Département des Sciences des Denrées alimentaires > Microbiologie des Denrées alimentaires
Chahed, Amina; Ecole nationale vétérinaire d'Alger
Jolly, Sandra ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie générale et autopsies
Vindevogel, Henri ; Université de Liège - ULiège > Département des Sciences des Denrées alimentaires > Hygiène des Denrées alimentaires
Language :
English
Title :
An Efficient Sampling Technique Used to Detect Four Foodborne Pathogens on Pork and Beef Carcasses in Nine Belgian Abattoirs
Publication date :
1998
Journal title :
Journal of Food Protection
ISSN :
0362-028X
eISSN :
1944-9097
Publisher :
International Association for Food Protection, United States - Iowa
Anderson, M.E., Huff, H.E., Naumann, H.D., Marshall, R.T., Damare, J., Johnston, R., Pratt, M., Evaluation of swab and tissue excision methods for recovering microorganisms from washed and sanitized beef carcasses (1987) J. Food Prot., 50, pp. 714-743
(1993) Microbiologie Alimentaire - Méthode de Routine pour la Recherche des Salmonella, , NF V 08-052. AFNOR, France
(1993) Microbiologie Alimentaire - Recherche de Listeria Monocytogenes - Méthode de Routine, , NF V 08-055. AFNOR, France
(1996) Microbiologie des Aliments - Méthode Horizontale pour la Recherche de Campylobacter Thermotolérants, , NF ISO 10272, V 08-026. AFNOR, France
Ayres, J.C., Microbiological implications in the handing, slaughtering, and dressing of meat animals (1955) Adv. Food Res., 6, pp. 109-161
Bean, N.H., Griffin, P.M., Foodborne disease outbreaks in the United States, 1973-1987: Pathogens, vehicles, and trends (1990) J. Food Prot., 53, pp. 804-817
Belongia, E.A., MacDonald, K.L., Parham, G.L., White, K.E., Korlath, J.A., Lobato, M.N., Strand, S.M., Osterholm, M.T., An outbreak of Escherichia coli O157:H7 colitis associated with consumption of precooked meat patties (1991) J. Infect. Dis., 164, pp. 338-343
Brackett, R.E., Presence and persistence of Listeria monocytogenes in food and water (1988) Food. Technol., 42, pp. 162-164
Buchwald, D.S., Blaser, M.J., A review of human salmonellosis: II. Duration of excretion following infection with nontyphi Salmonella (1984) Rev. Infect. Dis., 6, pp. 345-356
Buncic, S., The incidence of Listeria monocytogenes in slaughtered animals, in meat, and in meat products in Yugoslavia (1991) Int. J. Food Microbiol., 12, pp. 173-180
Butzler, J.P., Goosens, H., Glupczynski, Y., Helicobacter pylori, Campylobacter and other bacterial infections of the gastrointestinal tract (1992) Current Opinion in Infect. Dis., 5, pp. 80-87
Cohen, D.I., Rouach, T.M., Rogol, M., Campylobacter enteritis outbreak in a military base in Israel (1984) Isr. J. Med. Sci., 20, pp. 216-218
Dickson, J.S., Anderson, M.E., Microbiological decontamination of food animal carcasses by washing and sanitizing systems: A review (1992) J. Food Prot., 55, pp. 133-140
Dorsa, W.J., Cutter, C.N., Siragusa, G.R., Evaluation of six sampling methods for recovery of bacteria from beef carcass surfaces (1996) Lett. Appl. Microbiol., 22, pp. 39-41
Doyle, M.P., Escherichia coli O157:H7 and its significance in foods (1991) Int. J. Food Microbiol., 12, pp. 289-302
Duitschaever, C.L., Buteau, C., Incidence of Salmonella in pork and poultry products (1979) J. Food Prot., 42, pp. 662-663
Gill, C.O., Newton, K.G., The ecology of bacterial spoilage of fresh meat at chill temperature (1978) Meat Sci., 2, pp. 207-217
Grau, F.H., Prevention of microbial contamination in the export beef abattoir (1987) Elimination of Pathogenic Organisms from Meat and Poultry, pp. 221-233. , F. J. Smulders (ed.), Elsevier Science Publishing Co., Amsterdam. The Netherlands
Griffin, P.M., Ostroff, S.M., Tauxe, R.V., Greene, K.D., Wells, J.G., Lewis, J.H., Blake, P.A., Illnesses associated with Escherichia coli O157:H7 infections (1988) Ann. Intern. Med., 109, pp. 705-712
Griffiths, M.W., Listeria monocytogenes: Its importance in the dairy industry (1989) J. Sci. Food. Agric., 47, pp. 133-158
Harris, N.V., Thompson, D., Martin, D.C., Nolan, C.M., A survey of Campylobacter and other bacterial contaminants of premarket chicken and retail poultry and meats. King County, Washington (1986) Am. J. Public Health, 76, pp. 401-406
Hudson, W.R., Roberts, T.A., The occurrence of Campylobacter jejuni on commercial red meat carcasses from an abattoir (1982) Campylobacter: Epidemiology, Pathogenesis, Biochemistry, p. 273. , D. G. Newell (ed.), MTP Press, Lancaster, England
(1996) Micro-organisms in Foods 5. Microbiological Specifications of Food Pathogens, , Blackie Academic and Professional, London, England
Jouve, J.-L., Viandes et produits carnés (1996) La Qualité Microbiologique des Aliments: Maîtrise et Critères, pp. 201-335. , CNERNA-CNRS (ed.), Polytecnica, Paris, France
Lammerding, A.M., Garcia, M.M., Mann, E.D., Robinson, Y., Dorward, W.J., Truscott, R.B., Tittiger, F., Prevalence of Salmonella and thermophilic Campylobacter in fresh pork, beef, veal and poultry in Canada (1988) J. Food Prot., 51, pp. 47-52
Mafu, A.A., Higgins, R., Nadeau, M., Cousineau, G., The incidence of Salmonella, Campylobacter and Yersinia enterocolitica in swine carcasses and the slaughterhouse environment (1989) J. Food Prot., 52, pp. 642-645
McClain, D., Lee, W.H., Isolation and identification of Listeria monocytogenes from meat (1987) USDA, FSIS, Microbiol. Div., , Lab. Comm. no. 57 (9 September)
McMeekin, T.A., Microbial spoilage of meats (1982) Developments in Food Microbiology, 1, pp. 1-40. , R. Davies (ed.), Applied Science Publishing, London
Nortje, G.L., Swanepoel, E., Naude, R.T., Holzapfel, W.H., Steyn, P.L., Evaluation of three carcass surface microbial sampling techniques (1982) J. Food Prot., 45, pp. 1016-1017
Oosterom, J., Het voorkomen van Campylobacter fetus subspecies jejuni bij normale slachtvarkens (1980) Tijdschr. Diergeneesk., 105, pp. 49-50
Pierard, D., Van Damme, L., Stevens, D., Moriau, L., Lauwers, S., Detection of verocytotoxin-producing Escherichia coli in meat in Belgium (1994) Recent Advances in Verocytotoxin-producing Escherichia Coli Infections, pp. 77-80. , M. A. Karmali and A. G. Goglio (ed.), Elsevier Science Publishing Co., Amsterdam
Riley, L.W., The epidemiologic, clinical, and microbiologic features of hemorrhagic colitis (1987) Annu. Rev. Microbiol., 41, pp. 383-407
Roberts, T.A., Hudson, W.R., Whelehan, O.P., Simonsen, B., Olgaard, K., Labots, H., Snijders, J.M., Fournaud, J., Number and distribution of bacteria on some beef carcasses at selected abattoirs in some member states of the European Communities (1984) Meat Sci., 11, pp. 191-205
Stern, N.J., Green, S.S., Thaker, N., Krout, D.J., Chiu, J., Recovery of Campylobacter jejuni from fresh and frozen meat and poultry collected at slaughter (1984) J. Food Prot., 47, pp. 372-374
Stern, N.J., Hernandez, M.P., Blankenship, L., Deibel, K.E., Doores, S., Doyle, M.P., Ng, H., Westhoff, D.C., Prevalance and distribution of Campylobacter jejuni and Campylobacter coli in retail meats (1985) J. Food Prot., 48, pp. 595-599
Swaminathan, B., Link, M.A.B., Ayres, J.C., Incidence of salmonellae in raw meat and poultry samples in retail stores (1978) J. Food Prot., 41, pp. 518-520
Turnbull, P.C.B., Rose, P., Campylobacter jejuni and Salmonella in raw red meats. A Public Health Laboratory Service survey (1982) J. Hyg., 88, pp. 29-37
(1995) Pathogen Reduction
Hazard Analysis Critical Control Point (HACCP) Systems - Generic HACCP for Raw Beef, , U.S. Department of Commerce, Washington, D.C
Wells, J.G., Shipman, L.D., Greene, K.D., Sowers, E.G., Greene, J.H., Cameron, D.N., Downes, F.P., Wachmuth, I.K., Isolation of Escherichia coli serotype O157:H7 and other shiga-like-toxin-producing Escherichia coli from dairy cattle (1991) J. Clin. Microbiol., 29, pp. 985-989
